@juditvr

Proteomics, mass spectrometry, systems biology Professor at UW Genome Sciences Mother, runner, outdoor enthusiast

 Seattle, WA
Joined June 2011
  • Tweets53
  • Following74
  • Followers726
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Very proud of fearless graduate student @alexischang3000 for facilitating HT phosphotyrosine studies through this method.
Alexis Chang@alexischang300025 Apr 2023
Here's what happens when you start working with a postdoc & scientist too cheap to buy reagents: @MarioLeutert @RRodriguezMias Checkout our automated and cost effective phosphotyrosine enrichment protocol now in JPR! @juditvr Villen Lab, UW pubs.acs.org/doi/10.1021/acs…

Automated Enrichment of Phosphotyrosine Peptides for High-Throughput Proteomics

Abstract. Phosphotyrosine (pY) enrichment is critical for expanding the fundamental and clinical understanding of cellular signaling by mass spectrometry-b

pubs.acs.org
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How cells age? How they remain young? How they rejuvenate? We were amazed on how combining the multiple proteomic assays can yield mechanistic insight into the basic biology of aging , e.g. regarding asymmetric division, organelle partition, and trajectories of protein complexes.
Mario Leutert@MarioLeutert13 Mar 2023
Excited to share our newest #preprint: Multidimensional proteomics identifies molecular trajectories of cellular aging and rejuvenation. A great project with the @juditvr @DunhamLab @mkaeberlein teams! 📄Read: biorxiv.org/content/10.1101/… 💾Explore: rlsproteomics.gs.washington.… 1/9
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Excited to share our newest #preprint: Multidimensional proteomics identifies molecular trajectories of cellular aging and rejuvenation. A great project with the @juditvr @DunhamLab @mkaeberlein teams! 📄Read: biorxiv.org/content/10.1101/… 💾Explore: rlsproteomics.gs.washington.… 1/9
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Happy to share new preprint from superstar postdoc @MarioLeutert in my lab. One of a kind phosphoproteomic study (101 perturbations x 6) enabling cool analyses and novel discoveries in signaling. The regulatory landscape of the yeast phosphoproteome biorxiv.org/content/10.1101/…

The regulatory landscape of the yeast phosphoproteome

The cellular ability to react to environmental fluctuations depends on signaling networks that are controlled by the dynamic activities of kinases and phosphatases. To gain insight into these...

biorxiv.org
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I am very excited to share the latest technology from my lab. Proteome-wide identification of amino acid substitutions deleterious for protein function biorxiv.org/content/10.1101/…

Proteome-wide identification of amino acid substitutions deleterious for protein function

DNA sequencing has led to the discovery of millions of mutations that change the encoded protein sequences, but the impact of nearly all of these mutations on protein function is unknown. We addres...

biorxiv.org
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Mistranslation + biochem assays + MS = variant interpretation across the proteome. Innovative idea by @RRodriguezMias and co-developed with @noncanonikyle, and many other contributors from Villen, Fields and Noble lab. MiRo: MistranslationRocks
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I am missing #ASMS2021 this year. But several cool presentations from students and postdocs in my lab on phosphoproteomics, MS acquisition methods, and functional annotation of protein variants @AnthonyBarente @MarioLeutert @AlexHgO @smithianr @noncanonikyle
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Excited to share our review on PTM crosstalk @molcellprot with @sam_entwisle @juditvr ! We discuss the basic modules of PTM crosstalk and focus on emerging technologies to study PTM crosstalk proteome-wide! #Proteomics #TeamMassSpec doi.org/10.1016/j.mcpro.2021…
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"With science, numbers speak much louder than words." statnews.com/2020/05/19/vacc…

Vaccine experts say Moderna didn’t produce data critical to assessing Covid-19 vaccine

Several vaccine experts told STAT Moderna's Covid-19 vaccine data lacked critical context needed to assess efficacy.

statnews.com
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Judit Villen retweeted
Shao-En Ong@shaoenong3 Mar 2020
My lab is looking for new postdocs and research scientists (B.Sc) interested in kinase signaling, EMT, cancer, and proteomics. Please RT!
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Judit Villen retweeted
Lara Muffley@LaraMuffley4 Feb 2020
The Center for the Multiplexed Assessment of Phenotype is excited to announce the launch of our new #Internship! This will be a unique opportunity to work with a team of scientists at the cutting edge of genomics and proteomics. Learn more at cmap.gs.washington.edu/outre…
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01/2020 Issue --> embopress.org/toc/17444292/2… The cover (by @TeamSciStories) highlights two automated sample preparation methods for #phosphoproteomics -R2-P2 from @juditvr @uwgenome- & #clinicalproteomics -autoSP3 from J Krijgsveld @DKFZ- #proteomics
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Phosphoproteomics has catalogued 100,000s phosphosites. Can we pinpoint which are functional? We compare thermal stability of proteins and phosphoproteins to identify phosphosites altering structure and/or interactions. biorxiv.org/content/10.1101/…

Identification of phosphosites that alter protein thermal stability

Proteomics has enabled the cataloguing of 100,000s of protein phosphorylation sites [1][1], however we lack methods to systematically annotate their function. Phosphorylation has numerous biological...

biorxiv.org
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See also accompanying paper by @savitski_lab biorxiv.org/content/10.1101/…

Impact of phosphorylation on thermal stability of proteins

Reversible protein phosphorylation regulates virtually every cellular process and is arguably the most well-studied post-translational modification. Still, less than 3% of the phosphorylation sites...

biorxiv.org
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New automated end‐to‐end phosphoproteomic sample preparation method for multidimensional #CellSignaling studies --> bit.ly/2Mf31LL from @juditvr @uwgenome #proteomics #phosphoproteomics #MSBmethod
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Thesaurus, from @juditvr and colleagues: a new proteomics search engine that finds phosphopeptide positional isomers from PRM and DIA mass spec data. nature.com/articles/s41592-0…
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New preprint from the Villen lab: R2-P2 method for automated phosphoproteomic sample preparation from cell lysates and application to MAPK signaling; work led by @MarioLeutert with contributions from Noelle Fukuda and @RRodriguezMias biorxiv.org/content/10.1101/…

Automated high-throughput proteome and phosphoproteome analysis using paramagnetic bead technology

Recent developments in proteomics have enabled signaling studies where >10,000 phosphosites can be routinely identified and quantified. Yet, current analyses are limited in throughput, reproducibil...

biorxiv.org
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Facilitating phosphoproteomics analysis of hundreds of samples with robust and reproducible high throughout sample prep.
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We are recruiting for 2 postdoc positions for large scale studies of phosphorylation/ubiquitination or genotype to phenotype analysis. Programming experience is required but we welcome also applications for combined wet/dry positions embl.de/jobs/searchjobs/inde…
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New preprint from the lab @Miguel_MartinP in collaboration with the Kaeberlein lab @mkaeberlein: Protein kinase C is a key target for attenuation of Leigh syndrome by rapamycin biorxiv.org/content/10.1101/…

Protein kinase C is a key target for attenuation of Leigh syndrome by rapamycin

Leigh syndrome is a fatal neurometabolic disorder caused by defects in mitochondrial function. mTOR inhibition with rapamycin attenuates disease progression in a mouse model of Leigh syndrome (Ndufs4...

biorxiv.org
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