@f_fauser

Genome Engineer | Early R&D Scientist @SangamoTx | Postdoc @JonikasLab, @CarnegiePlants & @PrincetonMolBio | PhD @PuchtaLab, @KITKarlsruhe | Tweets are my own

 Fremont, CA
Joined September 2014
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  • Followers133
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We are thrilled to reveal @SangamoTx‘s Modular Integrase (MINT) platform at #ASGCT2024. Our MINT platform is a protein-guided genome editing method designed to facilitate targeted integration (TI) of synthetic DNA into chromosomal sites. PDF: sangamo.com/science/publicat… [1/10]
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🧬🚀 We’ve made substantial progress in development of our modular integrase, or MINT, platform- a gene editing method that we believe will enable large-scale genome engineering. Our updated manuscript which showcases these advances is available on bioRxiv bit.ly/41Y14ud
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Thrilled to share this update on bioRxiv: biorxiv.org/content/10.1101/… >10x activity, T cell data & genome-wide specificity evaluation!

Reprogrammed Serine Integrases Enable Precise Integration of Synthetic DNA

Despite recent progress in the ability to manipulate the genomes of eukaryotic cells[1][1]–[3][2], there is still no effective and practical method to precisely integrate large synthetic DNA constr...

biorxiv.org
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Friedrich Fauser retweeted
Martin Jonikas@JonikasLab19 Dec 2024
We are excited to share 71,700 new #Chlamy mutants from the CLiP2 collection pre-publication! Nearly-saturating genome coverage. Please note: new background strain, mating type, and antibiotic resistance. Read: biorxiv.org/content/10.1101/… Search and order: chlamylibrary.org/
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Martin Jonikas@JonikasLab10 Aug 2024
Excited to share our latest preprint! Biogenesis, engineering and function of membranes in the CO2-fixing pyrenoid biorxiv.org/content/10.1101/…

Biogenesis, engineering and function of membranes in the CO2-fixing pyrenoid

Approximately one-third of global CO2 assimilation is performed by the pyrenoid[1][1], a liquid-like organelle found in most algae and some plants[2][2]. Specialized membranes are hypothesized to...

biorxiv.org
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Thanks @FremontFire for putting this Niles Canyon fire out quickly and @FremontPD for patrolling the neighborhood :)
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After a mere blink in the cosmos -3 years as a preprint- our paper has found its home in @NatureComms . Thanks to all coauthors, the long supporting journey @Dinnenylab, and collaborators @JonikasLab and Michael Sussman. Oh, and by the way, I'm job hunting!!!
Josep Vilarrasa Blasi@pituvilarnadal20 Jul 2021
Preprint Alert!! We took a systems biology approach to establish #Chlamy as a simplified system to study osmotic stress pathways and identified conserved pathways across the green lineage, uncovering a new role for actin during osmotic stress acclimation, thread
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One of our lead scientists, @wraithnot is presenting @FASEBorg about our exciting Modular Integrase (MINT) platform, a new approach to 🎯 integration of large 🧬 constructs into precise locations within the human genome. #genomicmedicine #MINTplatform
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Systematic Development of Reprogrammed Modular Integrases Enables Precise Genomic Integration of Large DNA Sequences biorxiv.org/content/10.1101/…

Systematic Development of Reprogrammed Modular Integrases Enables Precise Genomic Integration of...

Despite recent progress in the ability to manipulate the genomes of eukaryotic cells[1][1]–[3][2], there is still no effective and practical method to precisely integrate large synthetic DNA constr...

biorxiv.org
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Friedrich Fauser retweeted
Jeff Miller@wraithnot17 May 2024
Our #MINTplatform does not use #CRISPR, #ZincFinger or #TALeffector to 🎯 DNA. Instead, we directly reprogram Bxb1 integrase to go where we want in the human genome. The details are in our @bioRxiv paper: bit.ly/3wwPLwy
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We are thrilled to announce the publication of our manuscript detailing the development of our Modular Integrase (MINT) platform #MINTplatform is now live on @bioRxiv! bit.ly/3wwPLwy @f_fauser @gdavis624 @arangundy @wraithnot
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Friedrich Fauser retweeted
Jeff Miller@wraithnot11 May 2024
Systematic Development of Reprogrammed Modular Integrases Enables Precise Genomic Integration of Large DNA Sequences biorxiv.org/content/10.1101/… - our paper describing reprogrammed serine integrases is now on BioRxiv!

Systematic Development of Reprogrammed Modular Integrases Enables Precise Genomic Integration of...

Despite recent progress in the ability to manipulate the genomes of eukaryotic cells[1][1]–[3][2], there is still no effective and practical method to precisely integrate large synthetic DNA constr...

biorxiv.org
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Tom Ellis@ProfTomEllis11 May 2024
Cool new preprint from Sangamo, engineering BxbI integrase to target native sites in the genome. Who needs landing pads now?
bioRxiv Synthetic Biology@bioRxiv_synbio11 May 2024
Systematic Development of Reprogrammed Modular Integrases Enables Precise Genomic Integration of Large DNA Sequences ift.tt/1aEgDKj #biorxiv_synbio
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MINT platform reprograms Bxb1 integrase for precise genome editing. Achieving up to 35% efficiency, it integrates kilobase-sized constructs into specific genomic sites, including human TRAC and AAVS1 loci.🧬👨‍🔬✂️#GenomeEditing #SyntheticBiology #GeneTherapy biorxiv.org/content/10.1101/…

Systematic Development of Reprogrammed Modular Integrases Enables Precise Genomic Integration of...

Despite recent progress in the ability to manipulate the genomes of eukaryotic cells[1][1]–[3][2], there is still no effective and practical method to precisely integrate large synthetic DNA constr...

biorxiv.org
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Now on bioRxiv: Systematic Development of Reprogrammed Modular Integrases Enables Precise Genomic Integration of Large DNA Sequences biorxiv.org/content/10.1101/… Original Thread: xitter.cf/f_fauser/status/178855…

Systematic Development of Reprogrammed Modular Integrases Enables Precise Genomic Integration of...

Despite recent progress in the ability to manipulate the genomes of eukaryotic cells[1][1]–[3][2], there is still no effective and practical method to precisely integrate large synthetic DNA constr...

biorxiv.org
Friedrich Fauser@f_fauser9 May 2024
We are thrilled to reveal @SangamoTx‘s Modular Integrase (MINT) platform at #ASGCT2024. Our MINT platform is a protein-guided genome editing method designed to facilitate targeted integration (TI) of synthetic DNA into chromosomal sites. PDF: sangamo.com/science/publicat… [1/10]
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Please find Greg Davis (@gdavis264), Sebastian Arangundy (@Arangundy), or myself (Poster 1680, Friday) during #ASGCT2024 to talk science; or meet up with Jeff Miller (@wraithnot) during FASEB in Rome! Thanks to everyone who contributed to this project – this was fun 😊 [9/10]
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Handle correction: @SArangundy
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And here is the bioRxiv link: biorxiv.org/content/10.1101/… Thanks to everyone who showed up at the #ASGCT2024 poster session. Didn't expect this to turn into ~5h of conversations :)
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Friedrich Fauser retweeted
Sangamo Therapeutics@SangamoTx9 May 2024
Today at #ASGCT2024 is the platform presentation for our next-generation Modular Integrase (MINT) technology, engineered to enable large-scale genome editing. Learn how the MINT platform utilizes Bxb1, to integrate large sequences of 🧬 into the genome. bit.ly/3QhlQyY
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Friedrich Fauser retweeted
Sangamo Therapeutics@SangamoTx9 May 2024
We are excited to announce next-generation Modular Integrase (MINT) technology, engineered to enable large-scale genome editing. The MINT platform is a versatile, protein-guided genome editing method designed to integrate large sequences of 🧬bit.ly/44GSRe4
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Last but not least, we demonstrated that our MINT platform is potentially compatible with AAV-mediated donor delivery and explored various AAV donor architectures. [8/10]
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