Bioinformatics PhD @ UCSD | Research Fellow @ Ragon Institute at MGH, MIT, and Harvard | glycan metabolism, gene expression, NGS, pathology (he/him)
Cambridge, MA
Joined September 2014
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1/5 Today, I’m sharing 7yrs of work. I believe we discovered a comprehensive mapping from protein structure to glycan structure, a genetic encoding for specific glycans, and a new paradigm in extracellular biology.
biorxiv.org/content/10.1101/…
#Glycotime
Seems like its finally happening. See you on the new platform. My handle and name are the same.
And honestly, its much more fun and much less icky there.
🦋🦋🦋🦋🦋
It takes two brilliant scientists like @hhefzi and @Nathan_E_Lewis to add fresh insight to a 100-year old phenomenon (subversion of the Warburg effect) AND turn that insight into a perfectly named tool (CHOZeLa) to transform biologics manufacturing.
Check out the preprint!
After ~8 years, happy to share work done w/@Nathan_E_Lewis showing you can eliminate the Warburg effect in mammalian cells-w/out impacting growth rate!-by simultaneous knock out of pyruvate dehydrogenase kinases and lactate dehydrogenases.
Thread below:
biorxiv.org/content/10.1101/…
Thanks to Philip Bartels, Thi Nguyen, and again Daniel Sandoval for chasing pathogenic variants with us.
Thanks to Meghan Altman, Isaac Shamie, @LCasalino88, @chem_christian, and @RommieAmaro for helping us explore the implications of GlycoTemplating in Influenza back when we could barely predict anything.
So why does it matter that glycans are genetically encoded?
Glycans can be >50% of protein’s weight, cover >50% of protein surface, and vital in immunology, metastasis, and autoimmune pathogenesis. Yet, many biologists ignored them because they are hard to measure and control
1/5 Today, I’m sharing 7yrs of work. I believe we discovered a comprehensive mapping from protein structure to glycan structure, a genetic encoding for specific glycans, and a new paradigm in extracellular biology.
biorxiv.org/content/10.1101/…
#Glycotime
Thank you to @shahriararab for support grafting our glycans onto the AF3 models!
With @GoogleDeepMind AF3 you can model protein structure, with the GlycoTemplate, you can model realistic glycoprotein surfaces. Together, we can change extracellular biology, drug design and development, and the landscape of biological inquiry.