@TimWelsh_

PhD candidate investigating protein phase separation and other small scale phenomena | @knowleslabcamb @Cambridge_Uni

 Cambridge, England
Joined October 2020
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  • Following89
  • Followers102
9Media
An amazing platform for ultrahigh throughput protein solubility screening! Check the paper: pubs.acs.org/doi/10.1021/acs… Well done Nadia, Tomas, @OellerMarc, @HAusserwoeger, Aviad, @TimWelsh_ , Rungzhang, @DaoyuanQian219, @VendruscoloLab, Sormanni lab and others!

Multidimensional Protein Solubility Optimization with an Ultrahigh-Throughput Microfluidic Platform

Abstract. Protein-based biologics are highly suitable for drug development as they exhibit low toxicity and high specificity for their targets. However, fo

pubs.acs.org
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Check out the new DigitISA assay for sensing biomolecular complexes in solution! Well done @GeorgKrainer @KadiLiisSaar @TimWelsh_ @magdaczek @BJacquat and colleagues from Owens, Alberti and Klenerman groups! nature.com/articles/s41467-0…

Direct digital sensing of protein biomarkers in solution

Nature Communications - There are limitations with current protein sensing methods. Here the authors report DigitISA, a digital immunosensor assay based on microchip electrophoretic separation and...

nature.com
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Our paper on the digital sensing of protein:aptamer interactions is finally published! One platform shows the ability to sense everything from single proteins up to biomolecular condensates and exosomes, while elucidating many biophysical parameters. Congrats to all authors!
Georg Krainer@GeorgKrainer
7 Feb 2023
📢 Finally out now: Our paper describing DigitISA, a platform for direct digital sensing of protein biomarkers in solution 🧪 (incl. antibodies, amyloid aggregates & condensates). @KnowlesLabCamb @ChemCambridge Take a look: 👇nature.com/articles/s41467-0…

Direct digital sensing of protein biomarkers in solution

Nature Communications - There are limitations with current protein sensing methods. Here the authors report DigitISA, a digital immunosensor assay based on microchip electrophoretic separation and...

nature.com
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A new method for determining the interaction strengths among compounds that form biomolecular condensates relies on measurements of just one solute in one phase. Read go.aps.org/3Guq7L6 @DaoyuanQian219 @TimWelsh_ @AbellJonny @GeorgKrainer @MichaelsLabETH @KnowlesLabCamb
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While antibodies have a remarkable track record in therapeutics, achieving sufficient specificity remains an issue. A Review in @NatRevChem discusses the physicochemistry of non-specificity and outlines future opportunities to improve protein binding. go.nature.com/3O8fK1y
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En route to Heidelberg for the #EESPhaseSeparation conference. Only a few more countries to cross before Monday morning! Come find me all week if you want to chat condensates, microfluidics, or bikes!
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Check out our preprint discovering DNA-induced LLPS of antibodies based on surface patch driven non-specific interactions: biorxiv.org/content/10.1101/… Great work by @HAusserwoeger, @GeorgKrainer, @TimWelsh_, Tomas, @SchneiderMatth & Therese. Exciting collaboration with @novonordisk

Surface interaction patches link non-specific binding and phase separation of antibodies

Non-specificity is a key challenge in the successful development of therapeutic antibodies. The tendency for non-specific binding in antibodies is often difficult to reduce via judicious design and,...

biorxiv.org
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When theorists and experimentals collaborate good things happen @DaoyuanQian219. Excited to share our work on the measurement and mechanistic insights of tie-lines in biomolecular phase diagrams. Also, maybe we should stop calling PEG an "inert crowder"? biorxiv.org/content/10.1101/…

Tie-lines reveal interactions driving heteromolecular condensate formation

Phase separation of biomolecules can give rise to membrane-less organelles that contribute to the spatiotemporal organisation of the cell. In most cases, such biomolecular condensates contain...

biorxiv.org
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@KnowlesLabCamb #condensates
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More from @KnowlesLab at #bps2022 in #SanFrancisco. @eaandrzejewska @mus_greta, Alyssa & @TimWelsh_ presenting posters on pre-fibrilliar protein #aggregates and single-molecules studies, oligomer-membrane interactions, microfluidic spray-deposition & #biomolecular condensates.
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Super happy to have contributed to this fantastic piece of work investigating the underlying nucleation mechanisms in solutions of phase separating proteins! biorxiv.org/content/10.1101/…

Phase separating RNA binding proteins form heterogeneous distributions of clusters in subsaturated...

Macromolecular phase separation is thought to be one of the processes that drive the formation of membraneless biomolecular condensates in cells. The dynamics of phase separation, especially at low...

biorxiv.org
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Excited to share our recent work in @NanoLetters investigating the electrostatic forces that stablize protein condensates! pubs.acs.org/doi/abs/10.1021… Thanks to my amazing co-authors @GeorgKrainer, Jorge, @jerelleaj, @rcollepardo and more for their hard work! @KnowlesLabCamb

Surface Electrostatics Govern the Emulsion Stability of Biomolecular Condensates

Abstract. Liquid–liquid phase separation underlies the formation of biological condensates. Physically, such systems are microemulsions that in general hav

pubs.acs.org
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Pretty stoked to finish 4th overall (3rd male) in the British 24hr Cycling Time Trial National Championships Easily met my main goal of breaking the 55 year old Cambridge University record Taking a day to recover before training loads of new students on microfluidics this week!
Mersey Roads 24@MerseyRoads24
19 Jul 2021
Updated provisional overall result. Please let us know if any further issues.
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Final #transengland21 activity. @theracingcoll Will be back for more in the future and try to have less mechanicals... strava.app.link/CdvlUQUOEgb
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Finish 15:54. Brutal #transengland21
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CP5 robin hood's bay #transengland21
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CP4 - St Nicholas church #transengland21
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CP3 highest Inn in Britain #transengland21
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CP2 after some brutal gravel #transengland21
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