@StaphEric

Microbiologist, works on Staphylococcus aureus, quorum sensing, evolution and virulence. Views are my own. I don't always wear the flat cap.

Joined June 2013
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Staphylococcus aureus gliding comets: formation and observation - new observations of the S. aureus comets and how to get the assay to work biorxiv.org/content/10.1101/…

Staphylococcus aureus gliding comets: formation and observation

Staphylococcus aureus has been shown to move across soft agar surfaces by several different mechanisms. S. aureus can move using either spreading (a form of sliding motility producing generally...

biorxiv.org
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Mycobacterium smegmatis Expands Across Surfaces by Hydraulic Sliding - Environmental Microbiology Reports enviromicro-journals.onlinel…... - Our new paper is out!
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M. smegmatis uses new types of biofilm to enclose a liquid core and expand outwards in a weird new way.
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Artistic interpetation of a fluorescently tagged comet... Happy to talk to people about the S. aureus comet motility assay, if they need help with it etc
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S. aureus organises itself differently in motile comet tips and can go uphill
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Scientists present a soft #biorobotic heart to assess the feasibility and safety of new valve designs and #biomaterials to correct #TetralogyofFallot, offering a reliable model to inform reconstructive heart surgery. @ScienceTM scim.ag/7MM
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Study in #zebrafish shows the #heartbeat's mechanical forces remodel the developing heart's #extracellularmatrix. Image & research by Alessandra Gentile & @MargaAlbu et al @StainierLab @mpi_hlr @GunawanLab @wwu_muenster in @Dev_journal. On bpod.org.uk/archive/2024/7/2…
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Eric Pollitt retweeted
Development@Dev_journal4 Jul 2024
Llgl1 mediates timely epicardial emergence and establishment of an apical laminin sheath around the trabeculating cardiac ventricle Read this Research Article by Eric Pollitt @StaphEric, Emily Noël @noelresearchlab and colleagues @ScienceShef: journals.biologists.com/dev/…
Figure 1 A-J: Llgl1 promotes organised trabeculation and ventricular wall integrity. (A-J) Live light-sheet z-slices through the ventricle of Tg(myl7:LifeAct-GFP) transgenic wild-type and llgl1 mutant embryos visualising the myocardium at 120 hpf (A-E) and 80 hpf (F-J). Scale bars: 50 μm. Wild-type embryos have regular trabeculae emerging predominantly from the outer curvature of the ventricular wall at 120 hpf (A), whereas llgl1 mutant embryos exhibit disorganised trabeculae, ranging from wild-type-like (B), through to irregular trabecular CMs and multilayering of CMs (C-E). Some llgl1 mutants at 120 hpf exhibit apically extruding CMs (arrow, E). At 80 hpf in llgl1 mutants, CMs extrude apically from multiple locations in the ventricular wall (arrows G-J).

ALT Figure 1 A-J: Llgl1 promotes organised trabeculation and ventricular wall integrity. (A-J) Live light-sheet z-slices through the ventricle of Tg(myl7:LifeAct-GFP) transgenic wild-type and llgl1 mutant embryos visualising the myocardium at 120 hpf (A-E) and 80 hpf (F-J). Scale bars: 50 μm. Wild-type embryos have regular trabeculae emerging predominantly from the outer curvature of the ventricular wall at 120 hpf (A), whereas llgl1 mutant embryos exhibit disorganised trabeculae, ranging from wild-type-like (B), through to irregular trabecular CMs and multilayering of CMs (C-E). Some llgl1 mutants at 120 hpf exhibit apically extruding CMs (arrow, E). At 80 hpf in llgl1 mutants, CMs extrude apically from multiple locations in the ventricular wall (arrows G-J).

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Llgl1 mediates timely epicardial emergence and establishment of an apical laminin sheath around the trabeculating cardiac ventricle - our new paper in @Dev_journal is out now! journals.biologists.com/dev/… @noelresearchlab @deputy_derrick @J_SanchezPosada

Llgl1 mediates timely epicardial emergence and establishment of an apical laminin sheath around the...

Summary: The polarity protein Llgl1 promotes timely deposition of laminin on the apical surface of the developing cardiac ventricle through regulating timing of epicardial colonisation in zebrafish.

journals.biologists.com
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ICYMI
Darius Balciunas@Balciunas_Lab26 Mar 2024
1/8 Are you interested in knocking genes out in adult zebrafish? If so, you should read our new preprint! Our new ~ubiquitous ubbR:CreERT2 driver enables gene knockout in adult #zebrafish hearts with efficiency approaching 100%. Yes, we quantified it. biorxiv.org/content/10.1101/…

Highly efficient tamoxifen-inducible Cre recombination in embryonic, larval and adult zebrafish

We have generated transgenic lines containing zebrafish-optimized CreERT2 recombinase under the control of a recombinant ubbR promoter consisting of the zebrafish ubiquitin promoter supplemented with...

biorxiv.org
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We hope that many of its features can be utilised to understand morphology beyond the walls of the developing heart - so take it for a whirl, and let us know how you get on.
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The preprint describing the software, and how we used it to analyse zebrafish heart morphogenesis, are on bioRxiv tinyurl.com/mvjd45ze, while morphoHeart itself is available on GitHub github.com/jsanchez679/morph…
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morphoHeart is live! @J_SanchezPosada developed software for 3D visualisation and morphometric analysis of the heart including negative space segmentation of cardiac ECM volume. All packaged up into a handy GUI. #morphometrics #zebrafish
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A wee 2024 reminder that I'm looking for a job. Ideally it would be in the UK or Ireland (Scotland would be the dream). Please give me a heads-up if you have an opening coming up or know someone who has. You can check out my interests & CV at rosssmcinnes.github.io/ Cheers! 😃
Dr Ross McInnes@roscobacterium14 Nov 2023
My time at Birmingham will very sadly be ending in March. If you're looking for a dashingly handsome, well-spoken postdoc, that's not me, but I can do wet and dry lab ([functional]genomics, metagenomics, RNAseq) work in Gram-positives (especially Enterococcus) so keep me in mind.
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A real paradigm shift from Astrid Hendriks and @N_van_sorge on how to understand adaptive immunity to S. aureus IgM (not IgG) to wall teichoic acids predicts protection vs mortality in bacteremia—IgM superior in promoting opsonophagocytosis, including as a monoclonal therapeutic
Nina van Sorge@N_van_sorge17 Jul 2023
Did you ever wonder about the role of IgM in host defense against Staphylococcus aureus? In this preprint, we identify opsonic IgM to be critical for human immunity to S. aureus. Potential implications for antibody-based therapies and vaccines. biorxiv.org/content/10.1101/…

Glycan-specific IgM is critical for human immunity to Staphylococcus aureus

Staphylococcus aureus is a major human pathogen but the immune factors that protect against it remain elusive. In particular, high opsonic IgG titers achieved in preclinical S. aureus animal immuni...

biorxiv.org
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Eric Pollitt retweeted
70s Sci-Fi Art@70sscifi16 Jul 2023
Star Wars immunization promotional poster, 1979
A photo of C3PO and R2D2, with the caption "Parents of Earth, are your children fully immunized?"

ALT A photo of C3PO and R2D2, with the caption "Parents of Earth, are your children fully immunized?"

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One more #AskZebrafish 🤔 from Slack: What are the best antibodies for labeling zebrafish vasculature? We are talking antibodies and not transgenics 🙏
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Eric Pollitt retweeted
Tim Chico@Timchico13 Apr 2023
Fantastic work by Dr Ryan Snodgrass and many colleagues.
DMM Journal@DMM_Journal13 Apr 2023
Brand new article from @timchico & co from @sheffielduni on vascular abnormalities in #zebrafish model of haemorrhagic #telangiectasia that were prevented by synergistic inhibition of MEK & mTOR pathways, suggesting potential novel therapeutic approach 📰journals.biologists.com/dmm/…
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The method used to prepare Mycobacterium tuberculosis cells affects the bacterial cell wall, changing infection outcomes. elifesciences.org/articles/8…
Blue, red and yellow epifluorescence microscopy image

ALT Blue, red and yellow epifluorescence microscopy image

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