Let’s talk about visualizing cells! 👩🏽‍🔬🔬 We’re an online resource for open-access protocols, reagents, theory, and more. Brought to you by @STEMCELLTech.

Joined October 2019
Welcome to StainsFile by @STEMCELLTech! Here, you can enjoy easy and open access to all the protocols and supporting resources you need to help you fix, prepare, and stain specimens: stainsfile.com 👩🏽‍🔬💻🧫🔬 #cellstaining #histology #microscopy #microscopymonday
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New in #HumPathol: Routine prostate biopsy reports carry grade-specific tumor burden information beyond the highest Grade Group. sciencedirect.com/science/ar… #pathology #PathTwitter #PathX #GUpath
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Amino group 🤝 Carboxylic acid This is your sign to review your amino acids. From isoelectric point to accentuators, these basic building blocks play a huge role in staining. 🔖 Bookmark the guide so you're never caught mixing up glycine and alanine: bit.ly/4z6zlWh
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How do we create more efficient, scalable #histopathological workflows? 🪜This paper uses AI-based segmentation of H&E sections to provide researchers with a new step up. See the deep-learning framework for detecting occult cervical lymph node metastases: bit.ly/4hTXUjj
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StainsFile retweeted
Tuft cells in white for #TuftTuesday
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New tech could help move polarization imaging into routine diagnostic workflows. The prototype offers a label-free method for analyzing cancerous tissue, helping to reduce reliance on #staining in #histopathology. Learn more: bit.ly/3TYt7c3
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Got air-liquid interface or monolayer cultures in need of staining? Check out this protocol for whole-mount #immunocytochemical staining for almost any tissue type. In less than ten steps, you'll be ready for your session at the #confocal microscope! 🔬 bit.ly/3X4dQnR
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Single-cell master regulator analysis of multiple #PDAC cohorts identified malignant cells in three co-existing, molecularly distinct developmental lineage states, each with distinct histopathological morphologies and spatial architecture. Abstract: go.nature.com/3VXXnUU
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Pathology Visions is just over a week away in San Diego, CA! 🌴 Whether you are a seasoned clinician or a curious trainee, its not too late to join and learn about the future of digital pathology and AI. Learn more: bit.ly/4juTfp9
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Has a new semester brought new labmates to the bench? 🥽 Send them this resource for safety sheets and best practices when dealing with #histotechnology chemicals and reagents. Safety is no accident! bit.ly/3TTufO9
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Angiofibroma of soft tissue is a rare benign fibroblastic/myofibroblastic neoplasm. 🔍 This study identified a distinctive cytological features to support accurate diagnosis: vascular patterns within distinct stromal backgrounds. Learn more: bit.ly/4zvhp8K #Pathology
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This guide from @iHisto summarizes staining by organ across brain, lung, liver, tumor, and more, covering #H&E, special stains, IHC, multiplex IF, and ISH. Check it out to help you design your next staining session or improve your current workflows: bit.ly/4cKWKDR
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😵‍💫 Flummoxed by fixation? This protocol is optimized for whole-mount fixation of epithelial organoids for #immunostaining. It covers both dehydration and cross-linking methods for #ChemicalFixation, giving you more options with your samples: bit.ly/4y3xiBP
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Dr. Roger Li (@UrogerliMD) at @MoffittNews and his colleagues have published a comprehensive review of the most common #BladderCancer subtypes. They discuss the histological subtypes and divergent differentiation of urothelial carcinoma. Read the abstract now: bit.ly/4geHSjm
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🧠 The #BloodBrainBarrier poses unique challenges for #histotechnology. New research proposes an innovative histological workflow that enhances how researchers evaluate probe penetration and brain pathology. Learn more: bit.ly/4gQPrwJ
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New this month in JHC: "Spatial Proteomic Profiling, a Novel Method for Detecting Diagnostic and Prognostic Proteins in Pediatric Sarcoma." Click to read: journals.sagepub.com/doi/abs… #proteomics #sarcoma #immunohistochemistry
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Can you name all the different ways to stain #ElasticFiber? 🔖 From orcein dye to iron resorcin fuchsin to aldehyde fuchsin to iron hematoxylin and more, bookmark this resource to broaden your staining toolkit and hone your technique: bit.ly/4ymJIFd #Histology
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Dr. Lei Yan's group at @RushUniversity conducted a retrospective study to review the pleural fluid cytologies of patients diagnosed on pleural biopsy #histology. 📃 They found that a #mesothelioma diagnosis can be made from pleural fluid cytology samples: bit.ly/3Sm0R27
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💻 AI can analyze data, but it's the #histologists that understand the context. Dr. Quincy Gu gives a sneak peek of his keynote at the upcoming National Society for Histotechnology Convention and discusses "Intelligent Histology", which is the role AI will play in histology labs: bit.ly/3UDokg2
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New in #HumPathol: Co-occurrence of bronchiolar adenoma and lung adenocarcinoma: a study of nine cases revealing distinct clonal origins via integrated histologic, immunophenotypic and molecular analysis. sciencedirect.com/science/ar… #pathology #PathTwitter #PathX #pulmpath
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Collagen, smooth muscle, fibrin, & erythrocytes—using 2+ acid dyes can create vibrant, contrasting images to reveal numerous tissue components. Learn about #TrichromeStaining in this comprehensive guide, including methods and the science behind the stain: bit.ly/45OdJ4s
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