Every day is #RNADay

AWESOME CONFERENCE ALERT!! I had an absolute blast at the Fusion “Genome Regulation Through RNA” meeting back in 2024, and psyched to see its upcoming return! Let’s geek out about all things Chromatin and RNA together, in Cancun! Talk abstract deadline: 10/17 Details👇

Hello RNA World! Ever wonder what's "talking to" your favorite transcript, but were too scared to ask? In our review in @CellReports, @FKHM and I highlight new RNA-focused tools for discovering RNA interactions across organizational scales. Checkit! tinyurl.com/ydn6e3ac

And that's a wrap (for now)! I hope you've enjoyed this thread and give our preprint a read. Let us know what you think! (30/30)

Taken together, our data allow us to propose the first-ever model for the molecular architecture of a single-locus subnuclear compartment. This dramatically expands our understanding of the factory's functional scope, and its architectural disruption in DCM (24/30).

This explains some of our observations in the factory transcriptome, since many pre-TTN-proximal transcripts are putative targets of pre-TTN-proximal RBPs. Thus, these RNAs may be recruited via their interactions with factory-resident proteins (23/30).

In fact, nearly the entire pool of SAFB (an RBP and "nuclear matrix" factor) and of QKI (a splicing factor that's also mutated in DCM) are localized within TTN RNA factories! (22/30)

The factory proteome reveals a remarkable compartment that integrates RNA biogenesis with chromatin compartmentalization. Tons of exciting chromatin regulators (cohesins; SAFBs), RBPs (QKI; SRSF's; ELAVL1) and, surprisingly, DNA repair factors (FEN1; APEX1; MCM's) (21/30).

Factory localization also correlates with splicing efficiency, as TTN-proximal transcripts are more differentially spliced than other RNAs. Many alternative splicing events are defects (e.g. Retained Introns) specific to KO cells. Almost none are direct RBM20-targets (19/30).