@RevoluGen
revolugen.co.uk
Joined August 2019
  • Tweets218
  • Following165
  • Followers255
107Media
NEWS We are absolutely delighted to announce our agreement with #ProteoQuest for the distribution of Fire Monkey HMW-DNA extraction kits across #China including Hong Kong and Taiwan. User-friendly, single extraction for both @illumina @nanopore reads. revolugen.co.uk/news/37/111/…
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Low Molecular Weight mammalian DNA input ➡️Fire Monkey beads size selection/fragmentation workflow ➡️appx. 30Gb on a single LSK114/MinION run @nanopore
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Single extraction/fragmentation protocol (magnetic beads) => single (no nuclease flushes) mammalian genome run. 21kb and 22Gb on a MinION flow cell, LSK114 @nanopore
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White blood cells extraction/fragmentation magnetic beads protocol. 460+ pores sequencing, N50:21kb @nanopore LSK114/MinION.
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Fully automated Kingfisher @thermosci Fire Monkey beads HMW-DNA extraction from white blood cells. No size selection post-extraction LSK114/MinION @nanopore
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In development: Low molecular weight DNA extract input fragmentation on Fire Monkey magnetic beads for long @nanopore reads
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Fire Monkey chemistry + Dynabeads/Kingfisher Apex @thermofisher. 1ml E coli culture, on deck Proteinase K lysis to elution automated workflow. Raw data : 14Gb, read N50: 27kb on MinION/LSK114 @nanopore, single run.
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We've collaborated with @RevoluGen to deliver a fully automated protocol that produces HMW DNA for Nanopore sequencing in under 2 hours, representing a meaningful advance in sample preparation efficiency for long-read #DNAsequencing: bit.ly/3IfbZVV #genomics
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Results of collaboration with @LGCGroup demonstrating automated HMW-DNA extraction combining Fire Monkey chemistry + LGC magnetic beads + oKtopure liquid handling robot = superior HMW-DNA for short & long read #sequencing revolugen.co.uk/news/35/111/…
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Breakthrough bead-based automated, #HMWDNA extractions for high throughput long reads. #oKtopure instrument using Fire Monkey chemistry & @LGCGroup magnetic beads generates 21Gb of raw data, N50 ~45kb on @nanopore LSK114/MinION without post-extraction size selection. #Sequencing
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In development. Miniaturized and automatable. Beads fragmentation of low and high molecular weight DNA input @nanopore @PacBio @illumina
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Fire Monkey magnetic beads for mammalian samples. HMW-DNA or tuneable, miniaturized and automatable. No resting, fragmentation or size selection post-extraction. LSK114/MinION @nanopore performance.
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Miniaturized HMW-DNA extractions: 200microLt E coli culture + reduced Fire Monkey solution volumes on magnetic beads -> MinION/LSK114 @nanopore
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E coli Fire Monkey magnetic beads HMW-DNA extract captured on a precipitation star @nanopore
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100microLt whole blood, Fire Monkey magnetic, no size-selection post extraction, MinION LSKv14 @nanopore => ~8Gb and N50:40kb after 17hrs.
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Fire Monkey magnetic beads extract + Ligation Sequencing Kit V14/MinION @nanopore
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#GenomicsThailand 2023 #bentolab #revolugen #Vazyme
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Fire Monkey Spin columns & Magnetic Beads #HMWDNA #sequencing
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FM magnetic Total Nucleic Acid (HMW-DNA + RNA) protocols in development @nanopore @illumina
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At the #MDCU Faculty of Medicine at Chulalongkorn University, Medical Biochemistry Conference on this week - great attendance by professors, researchers, Ph.D. and master's degree students. #Bioentist #Revolugen #bentolab #TANbead #Vazyme #Acrobiosystem #MAGTEC
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