ALT Screenshot from page 1 of Replacing Animal Research’s Replacement Checklist. The text reads: "When looking for non-animal technologies, or alternative approaches to the use of animals in research, there are steps to take to thoroughly explore existing opportunities and new approaches to answering a research question. Similarly, for those reviewing proposals, whether an application for funding or for a licence to use animals in research, there are questions that do not require subject-specialist knowledge that could be asked to provide reassurance that the potential to replace animals has been fully explored. In the simplest terms, asking the What, Where, When, Who, How and Why questions should provide information to guide both researchers and reviewers of proposals and applications to ensure a thorough exploration of opportunities to avoid animal use has been performed."