Ultra-performance enzymes to power a new multiomic era
Branford, CT
Joined May 2024
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Long reads need long cDNA.
Join Jason Underwood, PhD, at the #ASHG2026 Omics Technologies poster session (Wed, Oct 21, 2:30–4:30 PM EDT) to see new long-read #RNAseq data from UltraMarathonRT®.
Then visit us at Booth #1722.
Learn more: bit.ly/4i68N0v
#RNA #ASHG26
ALT Join RNAConnect at ASHG 2026 to learn more about UltraMarathonRT RNA-Seq library prep kits.
Phosphorothioates protect therapeutic ASOs and siRNAs from nucleases. It turns out archaea make them naturally, with a single stereochemistry and dedicated writer enzymes. New in @CellCellPress from Maman et al.
Read the paper linked in the comments below.
1/ Out today in @CellCellPress 🎉
Collaborating across three continents to study how organisms at the extremes use RNA modifications, we discovered something new: the first natural modification of the RNA backbone, phosphorothioates (PS), and their writers. With @SchragaSchwartz
RNAConnect retweeted
1/ Out today in @CellCellPress 🎉
Collaborating across three continents to study how organisms at the extremes use RNA modifications, we discovered something new: the first natural modification of the RNA backbone, phosphorothioates (PS), and their writers. With @SchragaSchwartz
Bound gRNA can stop a conventional RT before it reaches your target, inflating apparent #CRISPR knockdown in an #RNA-targeting CRISPR system.
uMRT's strand displacement activity reads straight through it. Accurate KD/KO quantitation, any system, any amplicon location.
🔗⬇️
ALT Resolving RT-mediated Artifacts
Congrats to Leslie Watkins, Alan Zhu & Bin Wu (@HopkinsMedicine) on identifying a hidden #RTqPCR artifact that inflates apparent knockdown across RNA-targeting #CRISPR systems: Csm, PspCas13b & CasRx alike.
A discovery that helps the whole CRISPR community. 🎉
@binwu1
A pervasive RT–qPCR artifact inflates RNA knockdown by RNA-targeting CRISPR nature.com/articles/s41587-0…
We are exhibiting at #RNAFutures (@ucsc). Stop by our booth, and @JG_Underwood will talk through how to improve your RNA-seq by getting more genes and longer, more complete transcripts with #uMRT.
#RNA #RNAseq #DRS #transcriptomics
ALT RNAConnect is exhibiting at RNA Futures this week at UCSC.
New from Harvard's Churchman lab: AIR-seq marks newly made #RNA right inside standard #RNAseq libraries. No chemical conversion. No enrichment. Just RNA dynamics, resolved. Steady-state data is just a snapshot of the transcriptome, but AIR-seq can help make the movie.
🗞️🔗⬇️
RNA-seq tells us how much RNA is present in the cell. But to understand gene regulation, we need to easily measure the synthesis and decay rates driving this abundance. We introduce AIR-seq: analog intrinsic recoding sequencing. (1/6) biorxiv.org/content/10.64898…
Transcriptomics without killing the cell. POND-seq (@MolecularCell) harvests RNA from supernatant via engineered nanocages, enabling longitudinal profiling of the same cells over time.
Dream becomes methodology.
The snapshot just became a movie.
#RNA #RNAseq #Transcriptomics
Longitudinal monitoring of cytoplasmic RBP-RNA interactions and transcriptome in living cells by engineered protein nanocages dlvr.it/TTctdQ
Publicly available resource maps transcriptomic responses to 13,221 compounds. At 93,644 perturbations, rare transcript patterns identify cell states invisible at smaller scale. Comprehensive #transcriptomics as chemical biology infrastructure.
Worth reading and dreaming.
#RNA
Chemical-induced gene signatures (CIGS) is a high-resolution dataset of 319,045,108 gene expression events induced by chemical perturbation.
nature.com/articles/s41592-0…
ChemRNA (Nature Biomed Eng): 5′-OH-AUG-coding sequence-3′-OH. No cap. No UTRs. No poly(A). Translated. Immunogenic.
If non-canonical RNA species encode antigens, the immunopeptidome is larger than we thought.
Significant finding for cancer vaccine development?
#RNA #mRNA
Chemically synthesized, non-capped and non-polyadenylated peptide-coding RNA efficiently induces antigen-specific CD8+ T cells go.nature.com/4wtxIAz
At standard retroviral RT temps, 44–73% of a 7.6kb RNA degrades in 30 mins. Your #RNAseq workflow may be racing against hydrolysis…and losing.
New data at #RNATx '26 shows what a low-temperature, high-processivity RT looks like across SRS, LRS, and #DRS.
#RNA #NGS
ALT RNAConnect to present scientific poster at RNA Tx.
UltraMarathonRT® has peer-reviewed publications across 5 application areas on our website.
Find the work most relevant to your research, from rare #transcriptprofiling to #mods mapping, #shortread or #longread #RNAseq; access key research within just one click!
🔗below!
ALT Read UltraMarathonRT publications from PNAS, Nature, JMB, and RNA.
14,000+ isoforms. From one gene.
Dr. Graveley (UConn) used UltraMarathonRT® (uMRT)to sequence #Drosophila #DSCAM and paralytic, resolving #alternativesplicing and A-to-I #RNA editing combinations that no short-read method could.
See the data: bit.ly/4tNiZ1T
#RNASeq
ALT Direct RNA Sequencing of Dscam1 using UltraMarathonRT by RNAConnect
MMLVs stall on long, structured #RNA, and the mischaracterizations of the transcriptome often go undetected in your data.
We're presenting new data at #RNA2026 tonight! May 27, 8–10 PM
(ID# 672) Ultra long transcript identification in long-read RNA-seq with uMRT
@RNASociety