We're looking for a highly motivated Postdoctoral Fellow to join our team at IRB Barcelona to study MAPK signaling & conserved stress adaptation mechanisms, using cutting-edge transcriptomics, genomics & proteomics. Apply here until August 31st: recruitment.irbbarcelona.org…

Finally, inspired by the @JswLab, @josephmreplogle, we’ve used our data to assess the degree of transcriptional heterogeneity induced by each genetic perturbation. We found that the identity of the genetic drivers and the fitness consequences are condition-dependent.

Cell states can be genetically perturbed and contain information to infer gene function based on transcriptional phenotypes. Some mutants act as state attractors. For example, impaired mitochondrial function and endosomal sorting promote aging-like transcriptional phenotypes.

In addition, we have identified stress-primed cells whose expression program renders them with lower fitness in control conditions but grants them a stress-resistant phenotype.

For example, we disentangled the aged cell signature from bulk into different aging-associated expression patterns based on the expression of iron (FIT3) or hypoxia (HUG1) related genes. Cells that express any of those markers showed aged phenotypes.

This has led us to understand how transcriptional heterogeneity within a population establishes intrinsic and extrinsic cell states that reflect cell function. A plus is that you will be able to look at your favorite mutant single cell expression pattern.

To achieve this, we’ve created a new version of the Yeast Knock Out Collection that includes RNA-traceable genotype and clone barcodes using the original deletions.

One more Doctor in the house! Today, Hansol Bae succesfully defended her PhD thesis 🎉 Best of luck in your new future steps! 👩🏻‍🎓🍀

And pur best friends in the labs, the 1.5mL tubes fron @Eppendorf_Group We filled some of them with different buffers to build a colourful bridge Take a look again in case you could not spot it! instagram.com/p/C0uFL9RNx3Q/…

We always think of serological pipettes for changeing media or washing our plates... but what about a double-direction slider? We tries it out with @thermofisher pipettes and works pretty well!