@OBrien_lab

Vision research, electrical synapse, and vision restoration lab at the University of Houston.

 Houston, TX
Joined March 2020
  • Tweets63
  • Following241
  • Followers208
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The final version of our electrical synapse proteome paper is out in @eLife: Uncovering the electrical synapse proteome in retinal neurons via in vivo proximity labeling doi.org/10.7554/eLife.105935…

Uncovering the electrical synapse proteome in retinal neurons via in vivo proximity labeling

A proximity labeling screen uncovers the proteome of electrical synapses in retinal neurons.

elifesciences.org
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We captured proteins associated with Cx36 in mouse retina and with Cx35.1 in zebrafish retina to reveal an evolutionarily conserved core molecular machinery of electrical synapses. We identified a number of novel proteins and map the full life cycle of the connexins.
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This protocol paper is a nice companion to Stephan's whorl paper last fall in JBC. It's hard not to admire the beauty that comes from an intracellular trafficking defect.
Bio-protocol@bioprotocolbyte1 Aug 2024
Characterizing ER Retention Defects of PDZ Binding Deficient Cx36 Mutants Using Confocal Microscopy: bio-protocol.org/en/bpdetail… Work by Stephan Tetenborg, Elizabeth Martinez-Soler, and @OBrien_lab at @UHouston. #reproducibility #lifesciences #cellbiology
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John O'Brien Lab retweeted
IGJC2024@igjc202429 Jul 2024
Thank you Dr Stephan Tetenborg, great talk! "Trafficking of Connexin36 (Cx36) in the early secretory pathway"
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Honestly, isn’t this getting a little out of hand?
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Congratulations @EShihabeddin from @NeuroMDAUTH on his really cool new paper on methods to knock down genes in #zebrafish retina using strategies accessible to all. Thanks @NatEyeInstitute for F31 support. frontiersin.org/articles/10.…

Frontiers | Cost-effective strategies to knock down genes of interest in the retinas of adult...

High throughput sequencing has generated an enormous amount of information about the genes expressed in various cell types and tissues throughout the body, a...

frontiersin.org
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Stephan Tetenborg brings us a beautiful paper about how miss-trafficked #connexin 36 docks in the ER. Lovely CLEM and SBF by Viktoria Liss and Leo Breitsprecher and collab with @RZoidl. Check it out! sciencedirect.com/science/ar…
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See Chris Lee’s new paper examining calcium microdomains at #connexin 36 electrical synapses in the #retina. eneuro.org/content/10/8/ENEU…

Sources of Calcium at Connexin 36 Gap Junctions in the Retina

Synaptic plasticity is a fundamental feature of the CNS that controls the magnitude of signal transmission between communicating cells. Many electrical synapses exhibit substantial plasticity that...

eneuro.org
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Calcium regulates bidirectional plasticity of electrical synapses. Chris shows us that most electrical synapses experience transient calcium increases when puffed with glutamate, showing widespread potential for plasticity.
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Chris's paper makes use of a GCaMP-tagged Cx36 transgenic mouse that we developed. It expresses Cx36-GCaMP at electrical synapses in the retina, brain, and even the pancreas.
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A little eclipse action in Houston, TX
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Experience New Orleans at #ARVO2023. You will know who has by the powdered sugar on their pants.
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Congratulations @LisaOstrin for winning the Michael Oberdorfer Award in Low Vision Research. #ARVO2023
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When the OBrien lab leadership goes on vacation and doesn’t answer email, we REALLY go on vacation. I FOUND this tooth. What a thrill!
The Houston Museum of Natural Science ❤️🦖🧠🌌@hmns24 Jul 2022
Big, beautiful Allosaurus tooth found by a member of our Dino Dig 2022 crew. An amazing find 🦖🎉 #dinosaur #dinosaurs
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Zebrafish postdoc in the O'Brien Lab! Apply here or contact me directly: neurojobs.sfn.org/job/33697/…
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Woohoo! Congratulations @OBrien_lab grad student @EShihabeddin for a great score on your F31 application: Impact score: 20 Percentile: 5.0 Great job!
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Total lunar eclipse 05/15/22 from Houston, TX.
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Are you interested in #electricalsynapse? We are looking for a postdoc to work on #connexin niche specialization in #retina and brain using #zebrafish genetics in our new lab @UHouston. Vision scientist? Meet me at ARVO next week. Please RT.
Zebrafish rods and Cx35 gap junctions.

ALT Zebrafish rods and Cx35 gap junctions.

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Modular @NIH grant budgeting simplifies the budget process and sets a funding level target. When established, a modular R01 could pay for a research project. That is no longer the case; the cost of doing research has doubled. #RaiseTheModularLimit. 1/3
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This graph shows 1) NIH year 0 postdoc salary, 2) cost of 1 year 0 and 1 year 3 postdoc with benefits at UT Houston rates, and 3) full team including a technician and some PI salary, both estimated at 2/3 the cost of an average postdoc. #RaiseTheModularLimit. 2/3
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In 1999, the research team cost 49% of a modular R01; in 2013, 75%. In 2021, the same team cost 98%; there is no room for other costs. It is impossible to fund this smallest basic team with a modular R01. #RaiseTheModularLimit. 3/3
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