ALT EphB2 expression is upregulated in patients with SSc. (A) Heat map showing upregulation of EPHB2 (red square) in SSc skin patients compared to normal individuals. (B) EPHB2 transcript levels in the skin of normal individuals and SSc patients. Data was extracted from GEO GSE45485. (C) EPHB2 transcript levels in the skin of normal individuals and SSc patients extracted from GEO GSE130955 (normal = 33, SSc=48). The y-axis in C and B indicates the log2- signal intensity and transformed normalized count of EPHB2. (D) Correlation plot between EPHB2 transcripts count and mRSS from GEO GSE130955. (E) Representative dual immunostainings of EphB2 (red) and αSMA (green) expression in normal (n=10) and SSc skin (n=15) with DAPI nuclear staining (blue). Magnification 20x, scale bar 150µm. Quantification of EphB2 positive myofibroblasts per field.