Investigator with the National Human Genome Research Institute, in the NIH. Views are my own.
Bethesda, MD
Joined January 2016
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New from my lab: Ancestral sequence reconstruction analysis indicates the furin-cleavage-site insertion in the SARS-CoV-2 spike gene likely interrupted a codon academic.oup.com/ve/article/…
Meru Sadhu retweeted
New paper in #G3journal from @mongemmmm, @MeruSadhu, and colleagues presents a barcode-based high-throughput method that can track large numbers of independent replicates of a small number of combinatorial genotypes. buff.ly/Q0qgBQz
New paper from my lab! We describe our idea that high-throughput pooled experimental methods – typically used to test thousands of hypotheses at once – also have huge potential to help in “everyday” experiments testing one or a few focused hypotheses. doi.org/10.1093/g3journal/jk…
“High throughput” doesn’t have to mean hard/expensive! At its heart, the difference is that instead of picking a few clones of your transformation, you take all the colonies. And with low sequencing costs and the possibility of pooling sequencing, it will generally be affordable.
Meru Sadhu retweeted
New preprint from the lab: A maternal-effect toxin-antidote element causes larval arrest in C. elegans, by @sdraljefainc et al. biorxiv.org/content/10.1101/…
Meru Sadhu retweeted
Our obsession with yeast killer toxins was triggered by a collaboration with @MeruSadhu.
Now with @BPSIYork we have a PhD project to delve deeper into toxin effects (and defences) at the biophysical level.
Great opportunity for diverse skillsets. Apply:
tinyurl.com/y877rj3b
Now out in Genome Research! "Highly complete long-read genomes reveal pangenomic variation underlying yeast phenotypic diversity" genome.cshlp.org/content/33/…
Thanks again to Team Genome: Cory A. Weller, Ilya Andreev, Michael Chambers (@greenkidneybean), Morgan Park, Josh Bloom (@joshsbloom), and the NIH Intramural Sequencing Center
And thanks to Genome Research @genomeresearch and the anonymous reviewers - the entire process was very helpful and improved the manuscript substantially!
Lab news: Postbac Molly Monge is headed to Weill Cornell’s MD PhD program @TriIMDPhD! Congrats, Molly and Cornell!
Wooo, first paper from the lab! We discovered a yeast defense factor against the killer toxin K28: a previously uncharacterized gene we named KTD1. Check it out! Open access on PNAS: pnas.org/doi/10.1073/pnas.22…
Big congrats to Ilya Andreev, the postbac who fearlessly took on the project! And all the co-authors: Kamilla Laidlaw, Simone Giovanetti, Guillaume Urtecho (@goGUrtecho), Daniel Shriner, Josh Bloom (@JoshSBloom) and Chris MacDonald (@YorkYeast)
I’m excited to share the latest from my lab, now on the bioRxiv! We combined long-read genome sequencing with QTL mapping to understand how pangenomic variation causes phenotypic diversity in yeast. 🧵 biorxiv.org/content/10.1101/…
Big thanks to “Team Genome”! Cory A. Weller, Ilya Andreev, Michael Chambers (@greenkidneybean), Morgan Park, Josh Bloom (@joshsbloom), and the NIH Intramural Sequencing Center