ALT Deletion of P2y2r reduces cyst growth in a Pkd1 orthologous mouse model.

ALT Visual Abstract for the article titled "Loss of the Secretin Receptor Impairs Renal Bicarbonate Excretion and Aggravates Metabolic Alkalosis in Mice during Acute Base-Loading"

ALT SCTR KO mice display no acid-base disturbances and normal β-intercalated cell function during control conditions. A) Urine pH, HCO3-, titratable acid (TA), NH4+, and net acid (NA=(NH4+ +TA)-HCO3-) concentration in spot urine samples (n=10-12). B) 24h urine net acid excretion in metabolic cages (mean of two consecutive days, n=6-9). C) Venous pH and standard HCO3- concentration (n=15-16). D) Representative immunohistochemistry images stained for pendrin (brown), scalebars represent 50 µm. E) Pendrin protein abundance determined by immunoblotting (n=10-12). See Supplemental Figure 1 for full-length gel with sex indicated for each lane. F) Pendrin function as determined by the intracellular alkalization rate in intercalated cells from isolated perfused cortical collecting ducts after a luminal Clremoval (n=14-41 cells). Statistical differences were assessed by Student’s t-tests.