π€¦π»ββοΈπ§’πππ¬π¦ 𧫠Senior Scientist at UC San Diego department of Neurosciences
UC San Diego, Shaner Lab
Joined February 2019
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Australia, weβre coming for you π
W/ @NathanShaner
Gerard Lambert π³οΈβπ retweeted
Excited to share our recent paper published in @ACSSynBio on our work developing genetically encoded bioluminescent neurotransmitter sensors for glutamate! pubs.acs.org/doi/10.1021/acs⦠with @GElegans1 @NathanShaner @LABrechaun @_theglowup and many others who contributed.
HeLa cells are so awesome when stimulated with histamine π₯ΉThis is bioluminescence imaged on our Nikon Widefield w/ @NathanShaner @_theglowup β at San Diego, CA
Gerard Lambert π³οΈβπ retweeted
About 4 minutes of action from our best new intensiometric bioluminescent calcium indicator in HeLa cells exposed to l-histamine. Sorry for no time stamp or scale bar π₯Έ Seeing ΞL/L of >30-fold in some cells π w/@LABrechaun, contributions from @_theglowup, biorXiv soonπ€
I hope Anya Salih sees this photoβ€οΈ @SaliAnya remembering the fun times coral prospecting in Australia w/@NathanShaner β at San Diego, CA
Had fun imaging HEPG2 cellsπ the bottom cell has a lot of personality
w/ @NathanShaner #FluorescenceFriday β at San Diego, CA
Gerard Lambert π³οΈβπ retweeted
A great example of how important it is to get rid of #aberrations, to improve your #resolution, & to have a more sensitive #detection - True for telescopes as well as microscopes π¬πͺπ - Well done, @NASAWebb & @NASA! #imagequality
one of our summer student (not on Twitter) found this cool geometric actin structure in a U2OS cell π€©
#FluorescenceFriday w/@NathanShaner β at Poway, CA
Gerard Lambert π³οΈβπ retweeted
Happy #FluorescenceFriday! Check out this time-lapse movie showing the extrusion of larval epidermal cells during Drosophila development. Adherens junctions are visualized by E-cadherin-GFP.
@Dev_journal publication: doi.org/10.1242/dev.179606
Gerard Lambert π³οΈβπ retweeted
Uri Manor @manorlaboratory & I are organizing a new imaging course! Join us at @salkinstitute in San Diego this Aug to learn confocal microscopy and image analysis! Generously supported by @cziscience. Applications due July 1. #ImagingtheFuture salk.edu/confocal-microscopyβ¦
Soo cool πΈππΌππΌ β at San Diego, CA
Artificial #Cilia controls #Microfluidic flowπ
Individual Cilium Pt/Ti bilayered strip 50x5x0.01βΞΌm
Programmable arbitrary/switchable flow, 1 mm above ciliary metasurface, up to 60βΞΌm/s
Dr. Wei Wang, Qingkun Liu & Itai Cohen lab @Nature 2022
nature.com/articles/s41586-0β¦
Gerard Lambert π³οΈβπ retweeted
Happy #FluorescenceFriday! Look at that beautiful neural tube closing π. Imaged by the very talented @wang_jianxiong. #DevBio is alive and well @IMBatUQ.
Gerard Lambert π³οΈβπ retweeted
What is going on with these zebrafish basal skin cells? I invite you to a 𧡠on wound healing, hydraulic fracking, and macropinocytosis, from subcellular to tissue-scale! All part of my favorite PhD project, now up on bioRxiv (1/17)
biorxiv.org/content/10.1101/β¦
Gerard Lambert π³οΈβπ retweeted
We present light-sheet microscopy combined with structured illumination.
This enables rapid volumetric imaging in a light-sheet format with doubled resolution.
This will need a few tweets to discuss the details... 1/n
biorxiv.org/content/10.1101/β¦
Gerard Lambert π³οΈβπ retweeted
A great solution for targeted sequencing of Monkeypox is available and has been validated in the field in the past. Please reach out to me or our Infectious Disease team at Illumina for information on the panel.
With #monkeypox in the news, weβre looking back on this study using a pan-viral panel, designed by @Illumina in collaboration with #USAMRIID and synthesized by @TwistBioscience. #NGS @usamrdc
bit.ly/3wBvzWc
Gerard Lambert π³οΈβπ retweeted
Mysterious increase in the motility of these #lysosomes for this #FluorescenceFriday
@DavidMcGrath_9 did is as a control, but after adding a non-stimuli treatment lyso got crazy, maybe #mechanosignaling triggered by shear stress? IDK, but looksππ½!
@focalplane_jcs @cellcommlab
