@JesperOlsenLab

Mass spectrometry for quantitative proteomics. The Olsen Group @jespervolsen at NNF-CPR @UCPH_CPR , University of Copenhagen. 🦋 https://xitter.cf/t.co/aFgWn5C3jt

 Copenhagen
cpr.ku.dk/research/proteomics/olsen/
Joined October 2022
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JesperOlsenLab retweeted
KPL ApS@KPL_ApSJan 13
New publication alert!📢 KPL collaborates w/ @JesperOlsenLab on the first systematic comparison of LysC enzymes for proteomics published in @JProteomeRes A. lyticus LysC: >90% cleavage efficiency, >97% specificity Read more: pubs.acs.org/doi/10.1021/acs… #TeamMassSpec #proteomics

Comparative Analysis of Lysine-Specific Peptidases for Optimizing Proteomics Workflows

Abstract. This study presents a comparative analysis of three LysC endopeptidase homologues from Achromobacter lyticus (A. lyticus),Pseudomonas aeruginosa

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We present our collaborative project on “personalising clinical decisions in ovarian cancer through patient-derived in vitro models” for the #ERAPerMed video competition 🎬 Have a look at it 👇🏼 and don’t forget to suport us by putting like on Youtube 👍🏼 youtu.be/J4bc1MPqIqE?si=EkhR…

OVA-PDM - Personalising clinical decisions in ovarian cancer through patient-derived in vitro models

This video offers a clear, interactive overview of our collaborativ...

youtube.com
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🚀 Check out our preprint on the new Thermo Scientific Orbitrap Astral Zoom MS 💫👇🏼 biorxiv.org/content/10.1101/…

Higher-Throughput Proteome Profiling Enabled by Parallelized Pre-Accumulation and Optimized Ion...

High-throughput proteomics is critical for understanding biological processes, enabling large-scale studies such as biomarker discovery and systems biology. However, current mass spectrometry...

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The new Astral Zoom MS overcomes previous MS limitations and is now achieving ultra-fast scans up to 270 Hz. It allows to identity > 100,000 peptides with short LC gradients.
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With a 40% reduction in analysis time and >12,000 proteins covered in 2.7 hours, it's setting new benchmarks for proteome studies.
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🚨 Check out our new study on proteomic profiling in premetastatic colorectal cancer! Accross 412 tumors, we define molecular subtypes with distinct functional landscapes and revealed CAVIN1 as novel biomarkers for relapse risk. embopress.org/doi/full/10.10…

Proteomics of colorectal tumors identifies the role of CAVIN1 in tumor relapse

Molecular Systems Biology - Colorectal cancer molecular signatures derived from omics data can be employed to stratify CRC patients and aid decisions about therapies or evaluate prognostic outcome....

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The mesenchymal-like subtype was shown to be marked by increased CAVIN1 and PRELP. Higher CAVIN1 correlates with higher relapse risk in two independent cohorts. We confirmed this in a second cohort and were able to link this to Epithelial to Mesenchymal Transition.
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Knocking down CAVIN1 in a 3D model reveals its connection with tumour invasiveness. Hybrid-DIA based phosphoproteomics profiling reveals an intriguing link between mTOR-signalling and its potential association to CAVIN1.
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🚀 Excited to share our latest article in #singlecell proteomics published in Cell! SC-pSILAC allows to simultaneously measure protein turnover and abundance in single cells, unlocking the first large-scale, 2D proteomic insights at single-cell resolution! cell.com/cell/fulltext/S0092…

Global analysis of protein turnover dynamics in single cells

The SC-pSILAC method enables single-cell measurement of both protein abundance and turnover, providing notable advances in the depth and versatility of proteomic technologies.

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Histone turnover enables distinguishing between dividing cells from slow/non-dividing ones, due to the extremely slow core histone turnover in non-dividing cells.
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We confirmed this observation and exploited it to compare different modalities of cell division arrest in human foreskin fibroblasts.
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JesperOlsenLab retweeted
Shabaz Lab@ShabazLab7 Feb 2025
First announcement! The Brixen Summer School is back and is, as usual, building up a strong speaker list. Thank you @EuPAProteomics @msaid_de @thermofisher @biognosys @EvosepBio @SIGNATOPE_GmbH
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🚨 More research on single-cell proteomics! We evaluated how formaldehyde fixation preserve proteome state, drug response and cell integrity. Cell fixation can facilitate access to #singlecell by enabeling sample shiping and prolonged sorting 📦 pubs.acs.org/doi/10.1021/acs…

Formaldehyde Fixation Helps Preserve the Proteome State during Single-Cell Proteomics Sample...

Abstract. Mass spectrometry-based single-cell proteomics (SCP) is gaining momentum but remains limited to a few laboratories due to the high costs and spec

pubs.acs.org
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Check out the full thread on 🦋 jesperolsenlab.bsky.social
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🚨 Our #singlecell article is out! We describe the Chip-Tip label-free SCP workflow quantifying >5000 proteins in single HeLa cells. With high scalability, increased depth and throughput we can now envision large-scale LFQ-SCP biomedical studies! nature.com/articles/s41592-0…

Enhanced sensitivity and scalability with a Chip-Tip workflow enables deep single-cell proteomics

Nature Methods - Chip-Tip is a label-free quantification-based single-cell proteomics workflow for deep single-cell proteomics, which identifies over 5,000 proteins and 40,000 peptides in single...

nature.com
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Lastly we applied the workflow to analyse undirected stem cell differentiation enabling the accurate quantification of stem cells and lineage markers.
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Follow us on Bluesky! @jesperolsenlab.bsky.social🦉🦋
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