ALT (A) Triple contrast agent was injected intra-articularly into both knees of a cadaver, and the knees were imaged using a clinical CT with two energies (70 and 140 kVp) before injection, and at several timepoints after the injection up to 180-min timepoint. (B) The CT images were coregistered, and the cartilage was segmented from the 60-min timepoint images. Contrast agent concentrations in the segmented cartilage were calculated from the attenuation maps at different imaging energies. (C) After knee joint extraction, the obtained osteochondral samples were imaged in the air using µCT. Biomechanical mapping was conducted using indentation stress-relaxation protocol, and the equilibrium and instantaneous Young's moduli were calculated for the measured locations, and maps of the surfaces were created.