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ALT Fig. 4. Symptoms of black rot at the base of sweetpotato slips following exposure to soil–root inoculum treatments. Treatments are listed as soil condition × root status: A, C × H = clean soil with healthy roots; B, C × I = clean soil with inoculated roots; C, I × H = infested soil with healthy roots; and D, I × I = infested soil with inoculated roots. Red circles highlight A, healthy or B to D, symptomatic tissue at the slip base.

ALT Fig. 4. Sweetpotato genotypes and wild relatives harbor a compact NRC-H subclade. Maximum likelihood (ML) phylogeny of complete coiled-coil nucleotide-binding and leucine-rich repeat immune receptor (CNL) proteins identified across all 35 sweetpotato and wild relative genotypes, using NB-ARC domain sequences as predicted by NLRtracker. Branches predicted to correspond to major nucleotide-binding and leucine-rich repeat receptor (NLR) required for cell death (NRC) helper and sensor clades (NRC-H, NRC-S) were highlighted based on phylogenetic placement of NRC0/1 (helpers, purple) and Hero-A, Rpi-amr3i, and Bs2 (sensors, green), respectively. Tips linked to CNLs containing integrated domains are labeled with yellow dots. The purple phylogenetic tree (right) includes only sequences from the indicated NRC-H lineage (left), underlining the Ipomoea batatas, I. trifida, I. triloba, and I. littoralis sequences phylogenetically predicted as helper NLRs.


































