@GiessenLab

Structure, function, and engineering of protein assemblies @UMich. Microbial Biochemistry | Cryo-EM | SynBio | Encapsulins | Natural Products | Enzyme Filaments

 Ann Arbor
giessenlab.bme.umich.edu
Joined February 2020
  • Tweets49
  • Following514
  • Followers248
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Giessen Lab retweeted
Rezwan Siddiquee@RezwanSidd
Jan 14
Nature has a trick to evolve multimeric proteins via gene duplication. 🧬One copy compensates, the other explores.🧬 Although this has only been a theory so far. Until now: Directed evolution of multimeric proteins by dual-compensatory gene duplication. biorxiv.org/content/10.64898…
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New paper out @NatureComms. Tremendous work by postdoc Mike Andreas, congrats! nature.com/articles/s41467-0…
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New review out @ChemBioChem. Learn all about encapsulin shells! chemistry-europe.onlinelibra…

The Structural Diversity of Encapsulin Protein Shells

Encapsulin nanocompartments are one of the most diverse and widespread classes of prokaryotic protein compartments. This review focuses on the structural diversity of encapsulins with a focus on...

chemistry-europe.onlinelibrary.wiley.com
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New paper out at @acsnano. Awesome work by PhD student Seokmu Kwon (@smkwon6) and postdoc Mike Andreas! pubs.acs.org/doi/abs/10.1021…

Pore Engineering as a General Strategy to Improve Protein-Based Enzyme Nanoreactor Performance

Abstract. Enzyme nanoreactors are nanoscale compartments consisting of encapsulated enzymes and a selectively permeable barrier. Sequestration and colocali

pubs.acs.org
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Recent paper out at Protein Science (@ProteinSociety). Congrats to now graduated PhD student Robert Benisch (@robertbe_)! onlinelibrary.wiley.com/doi/…

Structural and biochemical characterization of an encapsulin‐associated rhodanese from Acinetobac...

Rhodanese-like domains (RLDs) represent a widespread protein family canonically involved in sulfur transfer reactions between diverse donor and acceptor molecules. RLDs mediate these transsulfurati...

onlinelibrary.wiley.com
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New preprint out @biorxivpreprint. Great work by PhD student Seokmu Kwon (@smkwon6) and postdoc Mike Andreas. Congrats! biorxiv.org/content/10.1101/…

Pore engineering as a general strategy to improve protein-based enzyme nanoreactor performance

Enzyme nanoreactors are nanoscale compartments consisting of encapsulated enzymes and a selectively permeable barrier. Sequestration and co-localization of enzymes can increase catalytic activity,...

biorxiv.org
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New preprint out @biorxivpreprint. First two-component encapsulin system. Incredibly complex assembly. Congrats to PhD student Cassie Dutcher and postdoc Mike Andreas! biorxiv.org/content/10.1101/…

A two-component quasi-icosahedral protein nanocompartment with variable shell composition and...

Protein shells or capsids are a widespread form of compartmentalization in nature. Viruses use protein capsids to protect and transport their genomes while many cellular organisms use protein shells...

biorxiv.org
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New preprint out @biorxivpreprint. First structure of a Family 2B encapsulin. Congrats to postdoc Mike Andreas! biorxiv.org/content/10.1101/…

The biosynthesis of the odorant 2-methylisoborneol is compartmentalized inside a protein shell

Terpenoids are the largest class of natural products, found across all domains of life. One of the most abundant bacterial terpenoids is the volatile odorant 2-methylisoborneol (2-MIB), partially...

biorxiv.org
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New paper out @NatureComms. Congrats to postdoc Mike Andreas, great work! nature.com/articles/s41467-0…

Cyclodipeptide oxidase is an enzyme filament

Nature Communications - Many cyclic dipeptide natural products can be modified by cyclodipeptide oxidase enzymes. Here, the authors report the structural characterization of the cyclodipeptide...

nature.com
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New paper out @NatureComms. Congrats to postdocs Jesse Jones (now faculty at Idaho State) and Mike Andreas! nature.com/articles/s41467-0…

Structural basis for peroxidase encapsulation inside the encapsulin from the Gram-negative pathogen...

Nature Communications - Peroxidase encapsulins are self-assembling protein compartments involved in oxidative stress response found in many pathogens. Here, the authors characterize the structural...

nature.com
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New publication from the lab. Congrats to PhD student @robertbe_ and postdoc Mike Andreas. Great work! science.org/doi/10.1126/scia…

A widespread bacterial protein compartment sequesters and stores elemental sulfur

An encapsulin protein compartment sequesters and stores elemental sulfur.

science.org
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Postdoctoral Research Fellow positions in cryo-EM | microbial biochemistry | protein engineering | natural product sciences available in our lab!
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1/ We’re excited to share our work on HIV-1 trafficking by dynein! Led by the amazing @badieyan in the lab with wonderful collaborations with @EdcampbellLab, @GiessenLab, Aiken Lab, Böcking Lab, and @morgan_desantis. TL;DR HIV-1 hijacks the dynein motor protein for motility 🧵
bioRxiv@biorxivpreprint
30 Aug 2023
HIV-1 binds dynein directly to hijack microtubule transport machinery biorxiv.org/cgi/content/shor… #bioRxiv
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Happy to have this paper published in @ACSSynBio doi.org/10.1021/acssynbio.2c… Congrats to my student @smkwon6

Engineered Protein Nanocages for Concurrent RNA and Protein Packaging In Vivo

Abstract. Protein nanocages have emerged as an important engineering platform for biotechnological and biomedical applications. Among naturally occurring p

pubs.acs.org
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Come join our faculty @UMich in beautiful Ann Arbor! Learn more and apply at jobs.sciencecareers.org/job/…
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New preprint is out on @biorxivpreprint: biorxiv.org/content/10.1101/… We characterize a highly acid-stable and dynamic encapsulin nanocage exhibiting pH-dependent all 'open' or all 'closed' pore states. #encapsulin #nanocage

Exploring the Extreme Acid Tolerance of a Dynamic Protein Nanocage

Encapsulins are microbial protein nanocages capable of efficient self-assembly and cargo enzyme encapsulation. Due to their favorable properties, including high thermostability, protease resistance,...

biorxiv.org
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New preprint is out on @biorxivpreprint: biorxiv.org/content/10.1101/… We engineer novel nanocages to package RNA and protein in vivo at the same time for future drug co-delivery applications. Congrats to my student @smkwon6 #RNA #drugdelivery #encapsulin #nanocage

Engineered protein nanocages for concurrent RNA and protein packaging in vivo

Protein nanocages have emerged as an important engineering platform for biotechnological and biomedical applications. Among naturally occurring protein cages, encapsulin nanocompartments have...

biorxiv.org
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Thanks to @GiessenLab and Prof Schiller @FRIAS_UFreiburg for the external examination
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Happy that our review on the structure, function, and basic biology of encapsulins is now available @AnnualReviews of Biochemistry: annualreviews.org/doi/10.114… #encapsulin #nanocompartment #stressresistance #naturalproducts

Encapsulins

Subcellular compartmentalization is a defining feature of all cells. In prokaryotes, compartmentalization is generally achieved via protein-based strategies. The two main classes of microbial protein...

annualreviews.org
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Giessen Lab retweeted
Yu Heng Lau@YuHengLau
7 Feb 2022
Our study on pore structure and molecular flux into #encapsulin nanocompartments with @GiessenLab is now in shiny typeset format in @ScienceAdvances science.org/doi/10.1126/scia… Congrats @nurentasneem, Lachlan, Regi, @taylorszyszka and Eric @MyxXanthus from our lab, and... 🧵
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