Assoc Professor @NUSingapore @ChemNUS Membrane/lipid biochemist. Educator who teaches chemistry in biology. Advocate for students in Faculty of Science.🇸🇬📯📯
Singapore
Joined April 2012
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Really excited to be sharing our newest work on the bacterial Tol-Pal complex! The amazing @yeowjiang solved cryo-EM structures of the TolQRA complex in 2 rotary states, aiding us to come up with a model for force transduction across the cell envelope! #SGBUG @SCELSE_SG @ChemNUS
Structural insights into the force-transducing mechanism of a motor-stator complex important for bacterial outer membrane lipid ... biorxiv.org/cgi/content/shor… #biorxiv_biophys
Shu-Sin Chng retweeted
We are hiring!
If you are a hardcore single-molecule biophysicist specialized in in vitro and in vivo fluorescence imaging, consider joining us! See the ad here: apply.interfolio.com/179992
Shu-Sin Chng retweeted
Congrats to Maggie, Andrea, Mia and Hanee for this work w/ collaborators Joseph and Matt Jorgenson!
Dysregulation of a nucleotidyltransferase induces division and surface glycan defects in Escherichia coli by altering related metabolite levels | PNAS pnas.org/doi/10.1073/pnas.25…
Shu-Sin Chng retweeted
Check out Shishen’s new work. @DuShishen The way FtsZ filaments are arranged still amazes me! 😍😍😍even after all these years.
1️⃣ Cover Story – ZapA and Bacterial Cell Division
Yuanyuan Cui et al. reveal how ZapA, a conserved divisome component, promotes FtsZ filament alignment and Z-ring formation in E. coli.
🔍 Molecular dynamics and implications for antibiotic design.
🔗doi.org/10.1002/mlf2.70037
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Cell cycle and morphogenesis are metabolic decisions: We show cells dynamically rewire nutrient processing to fine-tune cytosolic redox to precisely control timing of Cell cycle entry, Progression and Proliferation Check preprint biorxiv.org/content/10.64898… #Metabolism #Development
Shu-Sin Chng retweeted
New #mBio study finds that long O-antigen #polysaccharides are a double-edged sword. While critical for protection against external host assaults, their transport and assembly onto the surface comes at the cost of compromising the outer membrane barrier. doi.org/10.1128/mbio.02518-2…
Shu-Sin Chng retweeted
Using multiple cryo-EM structures, we revealed how the periplasmic chaperone SurA dynamically changes its conformation to deliver substrates to the BAM complex.
Miyazaki et al., 2025, bioRxiv
biorxiv.org/content/10.1101/…
Shu-Sin Chng retweeted
細菌に必須の膜タンパク質 MurJ と、それを阻害するファージペプチドとの構造解析・第２弾！
ファージ由来の SglPP7 と LysM は、同じ Lipid II フリッパーゼ MurJ を標的にするが、結合様式は異なる。収斂進化のようだ。
Hosoda et al., bioRxiv (2025)
doi.org/10.1101/2025.10.15.6…
Structural basis for MurJ inhibition by phage lysis protein SglPP7 suggesting convergence biorxiv.org/content/10.1101/… #biorxiv_molbio
Shu-Sin Chng retweeted
New in #mLife: @hyq2015, @JilongQ & @DrTotsika explore how both overly stiff and overly fluid membranes impair biofilm growth in E. coli—revealing a “Goldilocks” fluidity zone.
@wileymicrobio
👉doi.org/10.1002/mlf2.70024
Shu-Sin Chng retweeted
Our MurJ/LysM paper has been published in Science Advances.
Kohga et al., Sci. Adv. (2025).
This study elucidates, through cryo-electron microscopy, the mechanism by which the small bacteriophage-derived protein LysM inhibits the lipid II flippase MurJ.
doi.org/10.1126/sciadv.ady80…
塚崎研では、現在、発表前の論文（投稿中）を3つbioRxivにアーカイブしています。
bioRxivは生物系研究を査読前に公開するサイトです。
最先端の研究にご興味あればぜひ！
doi.org/10.1101/2025.04.26.6…
doi.org/10.1101/2025.04.02.6…
doi.org/10.1101/2025.01.03.6…
#構造生命科学研究室 #塚崎研 #NAISTバイオ
We are back for #SGBUG for Oct, with Shaun Tan from Benson Chen's grp at @ASTARsg, and Jason Chen from Chris Sham's grp at @IDTRP_NUSMed, sharing about genetic manipulation in gut commensals, and glycoengineering in S. pneumoniae, respectively!
If you are attending the Great Wall Symposium happening this week in Sicily, do check out @WeiChuenPoh's poster on very interesting TolB variants he has found, which might help us think deeper about the function of the bacterial Tol-Pal complex! Poster 93!!
...by carrying out protein biochemical, cell biological, and live cell fluorescence imaging experiments. Associated structural analysis of the proteins by cryo-electron microscopy will be undertaken via collaboration with other workers. 3/3
Join the group of Prof Ben Berks to study the bacterial Type 9 Secretion System.
Build on our recent work (Nature (2018) 564:77, Nat Microbiol (2021) 6:221, Nat Microbiol (2024) 9:1089, bioRxiv (2025) biorxiv.org/content/10.1101/…)... 2/3
Helping Ben Berks @UniofOxford
with an ad.
Postdoc Research Associate in the Molecular Analysis of Bacterial Protein Transport
my.corehr.com/pls/uoxrecruit…
1/3
Shu-Sin Chng retweeted
Right now is the best chance the scientific community has ever had to end the artificial scarcity of academic journals.
Check out my new op-ed urging @NIH @NIHDirector_Jay to disallow taxpayer dollars towards journal publication fees — something both publishers and scientists have played a role in perpetuating.
The last day for public comment on this topic is Monday, Sept 15. It’s time to unleash science.
Links in 🧵
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Be our IMB colleague! Join us for cool science!
OPEN FACULTY POSITION, INSTITUTE OF MOLECULAR BIOLOGY, ACADEMIA SINICA, TAIWAN nature.com/naturecareers/job…
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Oxidative protein folding is a crucial process for #mycobacteria. Disulfide bonds are critical for stabilizing cell division, cell envelope biogenesis, and antibiotic resistance proteins in mycobacteria. doi.org/10.1128/mbio.01083-2…
Shu-Sin Chng retweeted
Our study where we develop EvoBind2: Design of linear and cyclic peptide binders from protein sequence information is now published!
nature.com/articles/s42004-0…
Shu-Sin Chng retweeted
#Newpaper Bacterial cell walls are polymers made of sugar backbones cross-linked with amino acid peptides. Recent work from Manjula Reddy's lab led by @shambhavi_garde highlights how the identity of the amino acid most proximal to the sugar backbone is crucial for bacteria. 1/2
Shu-Sin Chng retweeted
A collaborative paper with Manjula Reddy’s lab @ccmb_csir led by @shambhavi_garde is now online @PNASNews. @aakash0308 from our lab @IISERPune contributed with some LC-MS experiments reported in this study.
pnas.org/doi/10.1073/pnas.25…
