Cell Biology meets Immunology and Virology, spiced up with nanobodies
Bonn, Germany
Joined May 2018
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Late #WeekendRead: We describe a novel reporter to visualize endogenous #inflammasome assembly. Suitable for cell lines, primary cells, and tissues as well as quantification by #microscopy and #flowcytometry. Happy to share. biorxiv.org/content/10.1101/…
As #nanobodies enter pyroptotic cells (left), Lisa also tested if recombinant #nanobodies prevent pyroptosis when added to the medium during #inflammasome activation (right). They do!
As cells no longer die by #pyroptosis, stabilized, endogenous #GSDMD in the plasma membrane can even stained with antibodies for cleaved GSDMD.
Surprisingly, expression of antagonistic #GSDMD #nanobodies stabilized monomeric GSDMD NTD-mNeonGreen in the plasma membrane.
We had previously tried to use a cool tool from @inflammgenetics to visualize #GSDMD pores in living cells (mNeonGreen remains with the #GSDMD NT), but mostly found GSDMD in intracellular structures ...
We raised #alpaca #nanobodies against #GSDMD and found that two cytosolically expressed nanobodies completely shut down pore formation, #pyroptosis, and cytokine release after #inflammasome activation ....
Ich habe @DoktorWeigl in seinem Podcast #MedNight am @UniklinikBonn @UniBonn besucht.
Das Immunsystem: Unsere Ausrüstung gegen Erreger | Podcast Folge 9 [Prof... youtu.be/TK8sy16OiMU?si=zS7s… via @YouTube
Looking for an exciting #PhD or #postdoc opportunity? Come join our lab to study #NLRP1 #inflammasomes or #gasdermin pore formation using #nanobodies and other molecular tools at @IIIBonn @UniBonn @ImmunoSens. Please RT.
Little teaser: this is how #GSDMD localizes to the plasma membrane when oligomerization and pore formation is inhibited. @IIIBonn @UniklinikBonn
ALT THP-1 cells expressing fluorescent GSDMD and VHH GSDMD-1 were treated with NLRC4 trigger MxiH. Upon activation, the N-terminal domain of GSDMD localizes to the plasma membrane when pore formation is inhibited by antagonistic nanobodies.
Well-deserved!! Congrats @StephaniePfaen1 for the Loeffler-Frosch Award of the Society of Virology @GesVirologie #GFV2023. She also delivered a fantastic talk highlighting exciting science and important mentors and colleagues along the way!
