@DeepSeqNotts

Core Next Generation Sequencing facility at the University of Nottingham. Experts in Nanopore, Illumina and Sanger sequencing

 Nottingham, UK
nottingham.ac.uk/deepseq/
Joined August 2012
  • Tweets161
  • Following98
  • Followers648
39Media
Hey @nanopore we have a weird blocking pattern on our flowcell... can you help?
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Just a few flowcells to return to @nanopore
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We have an opportunity for someone to join our fabulous bioinformatics team - nottingham.ac.uk/jobs/curren… - you will get to work with @nanopore @illumina @bionanogenomics @10xGenomics and more! Please retweet and dm for info!
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Rapid #genome-wide copy number profiling using adaptive sampling on @nanopore by @mattloose and the amazing team @DeepSeqNotts Real potential for clinical application.
Matt Loose@mattloose
30 Mar 2022
This gif is from one sample of three run on a single @nanopore promethION flow cell. Frankly I'm super impressed at the performance though there is headroom for more improvements. We ran the same libraries as on gridION and have updated our preprint here. biorxiv.org/content/10.1101/…
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This gif is from one sample of three run on a single @nanopore promethION flow cell. Frankly I'm super impressed at the performance though there is headroom for more improvements. We ran the same libraries as on gridION and have updated our preprint here. biorxiv.org/content/10.1101/…
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Happy Christmas all!
Deep Seq@DeepSeqNotts
20 Dec 2019
Hey @nanopore we have a weird blocking pattern on our flowcell... can you help?
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We've put out a short preprint today showing some updates in Readfish for @nanopore adaptive sampling. biorxiv.org/content/10.1101/… @alexomics is giving a talk shortly at the #nanoporeconf about this - but a short thread here.
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Earlier this week we put out a couple of preprints (doi.org/10.1101/2021.09.10.4… doi.org/10.1101/2021.09.13.4…) describing minoTour github.com/LooseLab/minotour…, an @nanopore tool we have been developing for several years. A bit of a 🧵
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I still remember the first time I saw data from @scalene showing ultra long reads (N50>=100kb) were possible. Since then we and others (especially @DrT1973) have worked to get longer reads. This weekend we tested the @circulomics @nanopore ultra long kits. Wow! 19.49 Gb N50 143kb
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Well @nanopore - always seems to happen this time of year... bit of a bug in readfish but sure we will sort it out soon....
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Well @nanopore - always seems to happen this time of year... bit of a bug in readfish but sure we will sort it out soon....
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So - our ReadFish paper came out on Monday nature.com/articles/s41587-0… using GPU - but what if you only have a laptop? Here's an update from @alexomics to our tools that enable ReadFish on Mac, Linux or Windows with no additional GPU or compute. looselab.github.io/2020/12/0… for details.
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One for #nanoporeconf
Matt Loose@mattloose
2 Dec 2020
So - our ReadFish paper came out on Monday nature.com/articles/s41587-0… using GPU - but what if you only have a laptop? Here's an update from @alexomics to our tools that enable ReadFish on Mac, Linux or Windows with no additional GPU or compute. looselab.github.io/2020/12/0… for details.
Many application spin out of this on the fly selective sequencing. Tunable custom target panels to genome gap filling..... things get very interesting from here.
Matt Loose@mattloose
30 Nov 2020
Great to see this paper out - loads of hard work from @alexomics and many others to get this completed - adaptive sampling on @nanopore sequencers targeting hundreds of genes to depths in excess of 40x on a single flowcell. nature.com/articles/s41587-0…

Readfish enables targeted nanopore sequencing of gigabase-sized genomes

Nature Biotechnology - A nanopore sequencer achieves selective sequencing of any region in the human genome.

nature.com
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Great to see this paper out - loads of hard work from @alexomics and many others to get this completed - adaptive sampling on @nanopore sequencers targeting hundreds of genes to depths in excess of 40x on a single flowcell. nature.com/articles/s41587-0…

Readfish enables targeted nanopore sequencing of gigabase-sized genomes

Nature Biotechnology - A nanopore sequencer achieves selective sequencing of any region in the human genome.

nature.com
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Announcing the Visium Spatial Gene Expression for FFPE Scientific Challenge! Share how you’d use Visium Spatial to unlock the full story of your FFPE tissue samples to win an experiment performed by us. Enter by 12/23. Learn more: bit.ly/2IIPjlk #spatial
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About to start out 100th @nanopore run for covid samples with the team from @DeepSeqNotts . A sad milestone but amazing work by our local team including many from @TeamNUH and @PostdocPat all in support of the work of @CovidGenomicsUK .
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Massive achievement for @TeamNUH Microbiology lab, one of the first labs in the UK to obtain UKAS accreditation for SARS-CoV-2 PCR. A true testament to the hard work & dedication of the micro team, maintaining <24-hr TAT & high quality, end to end service for our patients.
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🧬 The total number of #COVID19 sequences now released is 16,380. The sequences and analysis are freely available to view 👉 cogconsortium.uk/data
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Fantastic to be a part of this innovative distributed network.
This Post is from an account that no longer exists. Learn more
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Just shy of 800 sequences contributed to @CovidGenomicsUK by our team since this all began. Thanks to all involved from @UoNLifeSci and @nottmhospitals
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