1/2 We’re excited to announce our Industry Session keynote for the Immunity by Design: In Vivo Immune Cell Engineering Conference! Rachael Nimmo (former director of the Cell Technology Group at OXB).

1/2 The Immunity by Design: In Vivo Immune Cell Engineering Conference presents the Industry Session, featuring Lorenz Mayr (BioMedTech Consulting) Cristiana Pires (@AsgardThx ), and Sara Mangsbo (@uppsalauni).

1/2 The Immunity by Design: In Vivo Immune Cell Engineering Conferenceintroduces Session 3: Delivery Technologies & Translation, joining Alexandra Teleki (Uppsala University), Jai Prakash (Radboud University Medical Centre), and Samir El Andaloussi (Karolinska Institutet).

10) Combining the delivery of PIB mRNAs with miRNA mimics showed cooperative effects and initiated non-viral cDC1 reprogramming.

9) Importantly, miR-142 improved cancer cell reprogramming and enhanced anti-tumor immunity in vivo, leading to reduced tumor growth and increased survival rates.

8) Functionally, miR-142-enhanced cells produced more IFN-λ1, a cytokine strongly associated with cDC1 biology and anti-tumor immunity. We further identified TGFBR1 as a miR-142-regulated barrier to cDC1 reprogramming.

7) We then analyzed contribution of canonical and non-canonical miRNA products to cDC1 reprogramming. While miR-124-3p recapitulated miR-124’s effects, canonical miR-142 strands did not enhance cDC1 reprogramming. Instead, multiple 5′ isomiRs cooperated to achieve the phenotype.

6) Meanwhile, miR-124 acts by increasing chromatin accessibility and creating a permissive environment for the reprogramming factors PU.1, IRF8 and BATF3 (PIB) to activate cDC1 programs.

5) Mechanistically, miR-142 enhances reprogramming fidelity, repressing fibroblast identity and strengthening cDC1-specific programs. This is highlighted by increased expression of key cDC1 markers, including XCR1.

4) Two clear hits emerged: miR-124, which increased reprogramming efficiency; and miR-142, which improved cDC1 lineage fidelity. And these effects were conserved across mouse and human systems.