@CarionHeloise

Ph.D. student in the Marraffini lab @RockefellerUniv | Formerly @UCSF @UCBerkeley | NSF GRFP Fellow

Joined April 2019
  • Tweets48
  • Following251
  • Followers135
2Media
It's up! Super excited to share our work highlighting the utility of 🔥phage accessory genes🔥 as a tool to elucidate the many layers of phage-host competition. "If you want to know what the host is doing, just ask its virus" - Sukrit, probably
Joe Bondy-Denomy@joeBondyDenomy11 Apr 2023
Years ago, we wrote a proposal about using phage anti-defense genes to discover new defense systems. The idea was to take advantage of anti-defense gene clustering (islands) to ask what the neighbors of known anti-RM/CRISPR genes are inhibiting. 1/5 biorxiv.org/content/10.1101/…

Parallelized screening of virus accessory genes reveals diverse defense and counter-defense...

Genomes of bacterial and archaeal viruses are replete with fast-evolving, uncharacterized accessory genes (AGs), most of which likely antagonize host defenses or other viruses. We developed a...

biorxiv.org
1
9
33
5,504
Grad student @CarionHeloise of the Marraffini lab was interviewed by @MolecularCell about her latest paper on the activation of bacterial programmed cell death by phage inhibitors of host immunity. Check it out here: cell.com/molecular-cell/full…

Meet the authors: Sukrit Silas and Héloïse Carion

We talk to authors Sukrit Silas and Héloïse Carion about their paper “Activation of bacterial programmed cell death by phage inhibitors of host immunity” (in this issue of Molecular Cell) and the...

cell.com
2
4
1,020
Héloïse Carion retweeted
Ben Adler
@ben_a_adler26 Feb 2025
It is finally out! Muntathar Al-Shimary, Doudna Lab, @bradyfcress and I present a gene knockdown tool that works in diverse phages: CRISPRi through antisense RNA Targeting (CRISPRi-ART)! Check out the final paper in @NatureMicrobiol nature.com/articles/s41564-0…

CRISPRi-ART enables functional genomics of diverse bacteriophages using RNA-binding dCas13d

Nature Microbiology - Leveraging RNA-targeting dCas13d enables selective interference with phage protein translation and facilitates measurement of phage gene fitness at a transcriptome-wide scale.

nature.com
3
46
2
125
9,287
phages are spooky
This Post is from an account that no longer exists. Learn more
1
1
12
1,168
Did you know that bacteria remember their past for many generations? In this new preprint from us (@VillaniAlexis and Senen Mendoza) and Banin lab (@ShmidovE ), we reveal the "how?" and "why?" underlying a long-standing mystery in bacterial memory. biorxiv.org/content/10.1101/…

Multigenerational Proteolytic Inactivation of Restriction Upon Subtle Genomic Hypomethylation

Restriction-modification (R-M) systems, present in most bacterial genomes, protect against phage infection by detecting and degrading invading foreign DNA. However, like many prokaryotic anti-phage...

biorxiv.org
2
53
3
159
19,111
Héloïse Carion retweeted
MDMLab@MDMlab_Paris22 Aug 2024
Excited to introduce AntiDefenseFinder - a free, open source tool that detects anti-defense systems in any genomic sequence. Available here to toggle on/off: defensefinder.mdmlab.fr/. Read below for our insights into the >47K detectable systems📈 tiny.cc/8xrizz
1
41
5
116
24,824
Héloïse Carion retweeted
Yuping Li@liyuping9273 Jul 2024
I am thrilled to start my lab as an assistant professor @biozentrum & @NCCR_AntiResist of @UniBasel_en this fall. I will continue my research interest in bacteria & jumbophage interactions. Equally thrilled to be closer to our families after 10+ years.
35
22
1
335
41,972
Excited to share my latest work from the @MarraffiniLab, in collaboration with @PhoebeARice, now online @Nature!! nature.com/articles/s41586-0… We report the discovery of DNA glycosylases that provide anti-phage defense in bacteria! A thread 🧵: 1/
9
31
3
121
17,063
New preprint from the lab, led by outstanding student Claire Kokontis! (@CKokontis) In this work Claire et al. discover Imp1-Imp6, phage proteins required for the selective import of proteins into the jumbo phage nucleus! Imp1 (below) is the special one. biorxiv.org/content/10.1101/…
2
35
5
121
20,231
Héloïse Carion retweeted
Ben Adler
@ben_a_adler1 Mar 2024
Hachiman is hot off the (preprint) presses! @owen_t_tuck and I in @doudna_lab present a model for how this broad-activity defense system recognizes genome integrity, a core feature of phage infection. Check out the preprint and Owen's thread for the news:
Owen Tuck@owen_t_tuck1 Mar 2024
New preprint! @ben_a_adler and I @doudna_lab, in collaboration with @Emy_nim and @JoePogliano, unveil a mechanism for Hachiman immunity. The punchline - Hachiman is a genome integrity sensor. @UCB_Chemistry @igisci @HHMINEWS biorxiv.org/content/10.1101/… 🧵 1/14

Hachiman is a genome integrity sensor

Hachiman is a broad-spectrum antiphage defense system of unknown function. We show here that Hachiman comprises a heterodimeric nuclease-helicase complex, HamAB. HamA, previously a protein of unknown...

biorxiv.org
1
12
1
59
10,240
Héloïse Carion retweeted
Aude Bernheim@AudeBer8 Dec 2023
Yesterday, i had the honor of becoming part of the first Scientific Council for the French President, among a group of 12 incredible scientists. A tad far from my 🦠, but hopefully will help a bit in fostering the role of science & knowledge in our society and democracy.
14
9
1
261
27,717
Héloïse Carion retweeted
Ben Adler
@ben_a_adler19 Sep 2023
I’m really excited to share our new pre-print “Genome-wide Characterization of Diverse Bacteriophages Enabled by RNA-Binding CRISPRi”, with co-first author Muntathar Al-Shimary @doudna_lab and the newly-minted @bradyfcress lab! biorxiv.org/content/10.1101/… 🧵🧵 1/

Genome-wide Characterization of Diverse Bacteriophages Enabled by RNA-Binding CRISPRi

Bacteriophages constitute one of the largest sources of unknown gene content in the biosphere. Even for well-studied model phages, robust experimental approaches to identify and study their essential...

biorxiv.org
13
70
6
188
49,243
Héloïse Carion retweeted
MDMLab@MDMlab_Paris14 Sep 2023
Happy to share our new preprint! We used viperins🐍, to understand the conservation and diversification of immune genes across domains of life. A deep dive in the evolutionary history of immune genes shared by prokaryotes and eukaryotes! biorxiv.org/cgi/content/shor…
1
49
7
131
69,722
Héloïse Carion retweeted
Tim Stearns@StearnsLab5 Sep 2023
Class picture time for incoming @RockefellerUniv grad student class!
2
5
55
10,156
Héloïse Carion retweeted
Erin Huiting@ErinHuiting12 May 2023
The increasing discovery of anti-phage bacterial immune systems (>100 in 5 years!) means an increasing diversity of phage activators. Here, @joeBondyDenomy and I review phage proteins as a prominent ‘pattern of pathogenesis’ and how they activate immunity. tinyurl.com/tmrpwrnk
3
41
2
110
14,601
Héloïse Carion retweeted
Matt@mtcjh4 May 2023
It's finally out! 🚀 Before doing a genome-wide screen in P. aeruginosa looking for immune systems, look in these two conserved anti-phage hotspots we named cDHS1 and cDHS2. academic.oup.com/nar/advance… Congrats to all the coauthors! @joeBondyDenomy @ErinHuiting @ARDavidson_UofT

Core defense hotspots within Pseudomonas aeruginosa are a consistent and rich source of anti-phage...

Abstract. Bacteria use a diverse arsenal of anti-phage immune systems, including CRISPR-Cas and restriction enzymes. Recent advances in anti-phage system d

academic.oup.com
Matt@mtcjh14 Nov 2022
Excited to share an exciting discovery which I think will help us understand the anti-phage arsenal of bacteria! biorxiv.org/content/10.1101/… 1/

Core Defense Hotspots within Pseudomonas aeruginosa are a consistent and rich source of anti-phage...

Bacteria use a diverse arsenal of anti-phage immune systems, including CRISPR-Cas and restriction enzymes. Identifying the full defense repertoire of a given species is still challenging, however....

biorxiv.org
20
3
56
17,800
BIG Congratulations to @Nicole_D_Marino who just signed her offer letter at UPenn @PennVet Pathobiology Departmant to start her own lab! The Marino lab opens Jan 1, 2024 focusing on new discoveries at the interface of phage and host immunity! s/o to Chair @BrodskyIgor
13
4
1
129
10,551
Héloïse Carion retweeted
David Bikard@dbikard20 Apr 2023
If like me you like to analyse genomics data in Jupyter notebooks I have something for you: dbikard.github.io/genomenote…
9
177
5
782
127,637
Héloïse Carion retweeted
Vivek Mutalik@vivek_mutalik20 Feb 2023
Super thrilled to share our new publication led by @ben_a_adler “Multicopy suppressor screens reveal convergent evolution of single-gene lysis proteins” on single strand RNA Fiersviridae #phages nature.com/articles/s41589-0…
8
44
2
165
40,942
Erin is a ⭐️!! What a fun story
Erin Huiting@ErinHuiting6 Feb 2023
Our latest CBASS story from the @joeBondyDenomy lab has arrived! 🙌Through a serendipitous collaboration with Yue Feng's lab, we uncovered an anti-CBASS protein that ‘sponges’ up a broad spectrum of cyclic dinucleotides. cell.com/cell/fulltext/S0092… 1/14

Bacteriophages inhibit and evade cGAS-like immune function in bacteria

Bacteriophages antagonize cGAS-like bacterial immunity by sequestering immune signaling molecules and acquiring capsid gene mutations.

cell.com
1
10
881
Load more