I make fluorescent indicators that dynamically monitor metabolites and signaling molecules in the cell. Now @KULeuven, previously in @DKFZ and @VIBLifeSciences.
Leuven
Joined April 2016
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🧪🧬 New preprint!
What if the same biosensor could reveal how cysteine is regulated across life domains?
We introduce Cystector, a genetically encoded fluorescent biosensor for real-time cysteine monitoring in living cells.
biorxiv.org/content/10.64898…
🧵A thread ⬇️
Want to monitor cysteine dynamics in real time and with subcellular resolution? Cystector plasmids are now on Addgene!
E. coli (His-tagged):addgene.org/250652/
Mammalian cells:addgene.org/250653/
Yeast:addgene.org/250654/
Yeast mitochondrial:addgene.org/250655
Across systems, Cystector enables:
• live-cell measurements
• subcellular resolution
• cross-domain comparisons
• minimal perturbation of metabolism
If you work on: #redoxbiology #metabolism #ferroptosis #mitochondria #biosensors
…and want to monitor cysteine dynamics in your system, Cystector might help (🧬 Plasmids soon available on Addgene!).
Happy to discuss applications or adaptations-DMs welcome!
Brandán Pedre retweeted
Check out our latest research from the VIB-VUB Messens Lab🥳 In collaboration with the ULB Gurzov lab, we've applied a fluorescent biosensor to assess glycolysis and specifically quantify fructose-1,6-bisphosphate in liver cells🧪🔬 Amazing work @Israelhabanero 👏
Brandán Pedre retweeted
Evidence-based sports supplements: A redox analysis
sciencedirect.com/science/ar…
In our recent review, we describe the redox properties of the sports supplements labeled as effective by the IOC
1.Bicarbonates
2.Beta-alanine
3.Caffeine
4.Creatine
5.Nitrates
6.Carbohydrates
7.Proteins
Brandán Pedre retweeted
Thanks a lot for the invitation. We were happy to explain why catenated sulfur chain species - supersulfides matter in biology! @STakaaki26424 @HozumiMotohashi @TetsuroMat25054
Small summary bellow:
Invited Review ‘New aspects of redox signaling mediated by supersulfides in health and disease’ Takaaki Akaike et al., Free Radic Biol Med online 9 Jul 2024 (
sciencedirect.com/science/ar…) @SFRR_Europe @SFRRAustralasia @SFRBM @elsbiomedchem
Excited to share my latest review on genetically-encoded fluorescent redox biosensors! 🧬✨🧪🔬.
Free to access until 23rd August following this link authors.elsevier.com/a/1jNDO…
Besides, this review discusses novel biosensor architectures based on chemigenetic fluorescent reporters, such as HaloTag, UnaG, or FAST. Hope you enjoy reading it as much as I enjoyed writing it! #FluorescenceFriday
Last but not least, I would like to thank @FWOVlaanderen and @SFRR_Europe for their support, as well as @GiuseppeValacc1 and Henry J Forman for their invitation to write this review.
Brandán Pedre retweeted
Whoop! Four brand new lifetime sensors based on mTurquoise2 by the team of Yasushi Okada
biorxiv.org/content/10.1101/…
