@BementLab

Transient contractile arrays. Part of the Center for Quantitative Cell Imaging (@UWCQCI) at UW-Madison. Tweets mostly by @ZacSwider and @AniMichaud1.

 Madison, Wisconsin
bement.molbio.wisc.edu
Joined March 2019
  • Tweets88
  • Following213
  • Followers705
44Media
Bement Lab retweeted
Zac Swider@ZacSwider26 Mar 2022
Any #microscopy friends have any suggestions for a water stage cooler/pump? I'm specifically interested in something to slowly flow water through a light sheet imaging chamber so it will need a very slow and consistent flow rate. Maybe @ZakSwartz ?
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Bement Lab retweeted
Zac Swider@ZacSwider13 Feb 2022
Finally, we identify and describe differences in the excitable F-actin dynamics found within the cleavage furrow to those found outside the cleavage furrow. The video below shows raw F-actin labeling in magenta and recent net F-actin assembly in green.
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Bement Lab retweeted
Zac Swider@ZacSwider13 Feb 2022
Once present, excitable F-actin waves undergo stereotypical changes that correlate tightly with markers for cell cycle entry and exit.
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Bement Lab retweeted
Zac Swider@ZacSwider13 Feb 2022
Our latest preprint from the @BementLab: Here we combined high speed live imaging with computational analysis to investigate the natural history of cortical excitability in developing frog embryos. The movie below shows an embryo labeled for F-actin. biorxiv.org/content/10.1101/…
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A short detour here to give a shout out to @AniMichaud1 of @BementLab who will talk about waves in oocytes during "Frontiers in Cytokinesis" on Wednesday at 3:03! Be sure to join both of us in the Rho GTPase poster session next week
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What a good looking group of Quantitative Cell Biologists! Then there’s ⁦@BementLab⁩ off to the side... thanks for putting together a fantastic Modeling Cell Biology Hackathon ⁦@WallaceUcsf⁩! #nsffunded #qcbnet
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Cortical actin waves in a dividing Xenopus laevis embryo. Acquired in collaboration with @henryhetired of the @huiskenlab at @Morgridge_Inst. Hoping to get two color next time, but feeling pretty good about our first attempt🤩!
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Very relevant work by @BementLab and Bieling Lab in @eLife 1. showing that RhoGDI extracts active #RhoGTPases from membranes in vivo 2. this is required to shape Rho activity patterns doi.org/10.7554/eLife.50471 1/2

Extraction of active RhoGTPases by RhoGDI regulates spatiotemporal patterning of RhoGTPases

Through visualization of directly-labeled RhoGTPase both in vitro and in vivo, RhoGDI is found to spatiotemporally regulate RhoGTPase activity through the extraction of active RhoGTPase.

elifesciences.org
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3. GDI binds Rho at the membrane before extraction (2-step extraction mechanism?) 4. also includes nice analysis of "activeness" of constitutively active Rho mutants (very useful reference) 2/2
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Bement Lab retweeted
MPI Dortmund@mpimoph28 Oct 2019
Extraction of active RhoGTPases by RhoGDI regulates spatiotemporal patterning of RhoGTPases -published by the Bieling lab & @BementLab in @elife - Congrats to @adriana_golding & Ilaria Visco et al # doi.org/10.7554/eLife.50471

Extraction of active RhoGTPases by RhoGDI regulates spatiotemporal patterning of RhoGTPases

Through visualization of directly-labeled RhoGTPase both in vitro and in vivo, RhoGDI is found to spatiotemporally regulate RhoGTPase activity through the extraction of active RhoGTPase.

elifesciences.org
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LOCI has a new YouTube channel! Check out some of our recent videos, such as this feature on the principles of Second Harmonic Generation microscopy: youtube.com/watch?v=kPBgeYCm… … Keep up to date with @UWMadisonLOCI by subscribing.
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Starting my journey to Woods Hole for #AQLM. So excited to meet everyone and immerse myself in microscopy!
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