Assistant Professor 👩🏼💻 @ErasmusMC Find me at 🦋 https://xitter.cf/t.co/eVJeIcMtR8
Rotterdam, the Netherlands
Joined February 2010
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🧬 How do immune disease-relevant variants affect gene regulatory networks in CD4+ T cells?
🧪🖥️ We coupled two large-scale CRISPRi screens (>4M cells) to map the downstream cascades of thousands of SNPs
More details below 🧵👇 or in the preprint 📖 tinyurl.com/variant2CRE2gene…
🎯 The results help resolve individual immune-related loci (TYK2, DEXI/CLEC16A) and combined disease-relevant biological processes
🦠 We observe shared core immune programs (resp. to virus, T cell activation), but also disease-specific enrichments (tight junction org. in celiac)
🤝 This was a truly collaborative effort bringing together experimental and computational expertise. Many thanks to the whole team, especially my co-lead @DewiMoonen; @SchraivogelS and @LarsMSteinmetz for excellent supervision; and @AndreasGschwind, Stefan Schrod and many others!
‼️ MAJOR UPDATE from the preprint on bioRxiv: we aim to distinguish cell type composition from intracellular effects by replicating our results in single cell RNA-seq from 1,139 donors. We prioritize 729 intracellular trans-eQTL that are likely intracellular.
